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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 Dec 1;90(23):10922–10926. doi: 10.1073/pnas.90.23.10922

Complex synthetic chemical libraries indexed with molecular tags.

M H Ohlmeyer 1, R N Swanson 1, L W Dillard 1, J C Reader 1, G Asouline 1, R Kobayashi 1, M Wigler 1, W C Still 1
PMCID: PMC47893  PMID: 7504286

Abstract

Combinatorial methods of chemical synthesis allow the creation of molecular libraries having immense diversity. The utility of such libraries is dependent upon identifying the structures of the molecules so prepared. We describe the construction of a peptide combinatorial library, having 117,649 different members, synthesized on beads and indexed with inert chemical tags. These tags are used as a binary code to record the reaction history of each bead. The code can be read directly from a single bead by electron capture capillary gas chromatography. We demonstrate the correct selection of members of the library on the basis of binding to a monoclonal antibody.

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Selected References

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