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Recombinant human RNASET2 was expressed in CHO cells, affinity purified on a Ni sepharose column and then separated by SDS-PAGE, blotted and detected with mouse anti-hrRNASET2 antibodies (12.5%). Lane 1 – MW marker; lanes 2,3,4 – three different batches of hrRNASET2. The band is in the range between ∼ 34-45 kDa indicating the size of the glycosylated protein.
