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. 2016 Mar 4;3(2):71–84. doi: 10.18632/oncoscience.295

Figure 2. RNase activity assay.

Figure 2

The RNase activity of purified hrRNASET2 was assayed by observing the change in fluorescence intensity of toluidine blue on binding with yeast RNA. hrRNASET2 samples (10 μl) were placed over a plate containing 10 ml 0.1% yeast RNA and 0.8% agarose, and incubated for 30 min (37°C). Agarose plates were stained with 0.02% (w/v) toluidine blue to detect RNase activity. RNase activity was quantified by placing samples (50 μl) in 50 μl 100 mM sodium acetate buffer (pH 4.5) that contained 4 mg/ml yeast RNA.