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. 2015 Nov 2;16(12):1330–1341. doi: 10.1111/tra.12337

Figure 1.

TRA-12337-FIG-0001-b

FHL4 and FHL5 patient CTL establish Syntaxin 11 and Munc18‐2 antibody specificity. A) Schematic representation of the STX11 and Munc18‐2 amino acid sequences indicating the effects of the homozygous mutations present in the FHL4 (847–1850) and FHL5 (UPN666) patient cells used in this study compared to healthy donor (HD). B) Western Blots on human CTL lysates against Munc18‐2 and STX11 using the two polyclonal rabbit antibodies 5182 and 5412. C) Percentage of P815 target cells lysis (% killing) for HD (solid black line, squares) or FHL5 patient UPN666 CTL (dotted black line, triangles) at Effector:Target ratios shown. CTL,10 days post restimulation. D) Granule release assay showing surface LAMP1 after 30 minutes PMA‐Ionomycin stimulation of CTL from HD (solid black) and FHL5 patient UPN666 (dotted black line). Solid gray line: unstimulated HD CTL. CTL, 10 days post restimulation.