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. 2015 Oct 30;6(41):43483–43495. doi: 10.18632/oncotarget.5820

Figure 4. CDK1, upstream kinase of NPM, enhanced HCC cell migration and invasion.

Figure 4

A. CDK1 was efficiently knocked down (C10 and C11) in Bel 7402 and SMMC with a lentiviral-based approach, confirmed by qPCR. B. Knockdown of CDK1 reduced NPM phosphorylation but not the total NPM when compared with NTC. C. BrdU cell proliferation assay demonstrated that Knockdown of CDK1 in Bel 7402 and SMMC cells (shCDK1-C10 and shCDK1-C11) significantly reduced cell proliferation rate with compared with control (NTC) (*p < 0.05, student t test). D. Cell migration and E. invasion assays demonstrated the number of migrated and invaded cells was significantly reduced in shCDK1-transfected Bel 7402 and SMMC cells (shCDK1-C10 and shCDK-C11) when compared with control (NTC) (*p < 0.05, **p < 0.01, ***p < 0.001, student t test) (magnification × 10).