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. 2015 Oct 27;6(42):44538–44550. doi: 10.18632/oncotarget.6253

Figure 5. miR-222 directly targets and reduces the expression of ABCG2 in TSCC.

Figure 5

A. Predicted target sequences for miR-222 are located in the 3′-UTR of ABCG2 mRNA. B. Dual luciferase reporter assays were performed to evaluatethe target gene of miR-222. Following transfection with pGL-ABCG2 for 24 h, luciferase activity was significantly reduced in cells treated with miR-222 mimics relative to cells treated with control mimics (*p < 0.05 vs. blank and control). After the seed region of the target site was mutated (pGL-ABCG2 m), the effects of miR-222 on luciferase activity were abolished. C. Differential expression of miR-222 was tested by quantitative qRT-PCR in UM1 cells transfected with control mimics or miR-222 mimics and UM2 cells transfected with control LNA or miR-222 LNA (*p < 0.05). D. UM1 cells displayed decreased levels of ABCG2, ERCC1 and Slug proteins following treatment with miR-222 mimics, and UM2 cells display increased levels of ABCG2, ERCC1 and Slug proteins following treatment with miR-222 LNA.