Table 1.

Relevant characteristics of the positive broad-range PCR samples

Case number	Sample type	16S rRNA identificationa	Hospital area	HAI	Symptomatology of HAI	Outcome
002-001	Bronchial aspirate	Mixed infectionb	ICU-A	Undemonstrated bacteraemia	Yes	Improvement
002-005	CSF	Agrococcus sp.	ICU-N	Meningitis	Yes	UD
002-011	Blood culture	Mixed infectionb	BU	Undemonstrated bacteraemia	Yes	Improvement
002-085	Blood culture	Pseudomonas sp.	Haematology	Bacteraemia	Yes	Death
002-088	Blood culture	Mixed infectionb	Haematology	Sample contamination	Yes	Death
002-095	Blood culture	Bacillus sp.	Haematology	Bacteraemia	Yes	UD
002-114	Blood culture	Mixed infectionb	Haematology	Bacteraemia	Yes	UD
002-174	Blood culture	Leuconostoc sp.	Haematology	Sample contamination	Yes	Improvement
002-028	Catheter tip	Staphylococcus sp.	BU	Catheter colonisation	Yes	Improvement
002-029	Catheter tip	Staphylococcus sp.	Cardiology	Catheter colonisation	Yes	Improvement
002-038	Catheter tip	Staphylococcus sp.	Cardiology	Catheter colonisation	Yes	Death
002-039	Catheter tip	Kocuria sp.	Haematology	Catheter colonisation	Yes	Improvement
002-167	Catheter tip	Enterococcus sp.	PIM	Catheter colonisation	Yes	Transfer
002-169	Catheter tip	Enterococcus sp.	Haematology	Bacteraemia	Yes	UD
002-023	Urine	Pseudomonas sp.	ICU-A	UTI	Yes	Death
002-065	Urine	Achromobacter sp.	Haematology	UTI	Yes	Death
002-103	Urine	Pseudocitrobacter sp.	ICU-A	Catheter colonisation	Yes	Death
002-104	Urine	Escherichia sp.	ICU-A	UTI	Yes	Improvement
002-105	Urine	Enterococcus sp.	Neurology	UTI	Yes	Transfer
002-106	Urine	Escherichia sp.	MMF	UTI	Yes	UD
002-170	Ulcer	Salmonella sp.	PI	Wound	Yes	UD
002-037	Wound secretion	Staphylococcus sp.	UTIP	STI	Yes	Death
002-171	Wound secretion	Enterococcus sp.	Rheumatology	Wound	Yes	Transfer

CSF cerebrospinal fluid; ICU-A intensive care unit, adult; ICU-N intensive care unit, new-born; BU burn unit; PIM paediatric internal medicine; MMF maternal foetal medicine; ICU-P intensive care unit, paediatric; UTI urinary tract infection; PI paediatric infectology; STI soft tissue infection; HCAI clinical healthcare infection; UD the outcome was not known at the time of the study

^aThe species designation was determining with the closest genetic identification based on GenBank sequence analysis (Fig. 1)

^bAetiological agents of polybacterial infections could not be identified due to low-quality sequences