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. 2015 Oct 9;2(11):1697–1704. doi: 10.1016/j.ebiom.2015.10.012

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© 2015 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Optical microscopy (OM), TEM, and HRTEM micrographs of lung cells from Parisian asthmatic children. (a, b) OM after Diff-Quick staining (magnification 100 ×) showing (arrows) lung cells containing black material. (c) TEM micrograph of a lung cell (likely type II pneumocyte) laden with black material mainly composed of lamellar bodies filled with concentric phospholipid layers of oligolamellar surfactant as evidenced by (d) TEM and (e, f) HRTEM of a black spot (the interlayer spacing is approximately 2 nm).