The data were also fitted using a model in which IκBα (
A) does not act as a transcriptional repressor in the feedbacks nor (
B) in the expression of each single gene, while the role of NF-κB as transcriptional activator (green arrows in (
A)) is preserved. (
C) Gene expression data from the experiment in
Figure 6 were fitted using the models of (A) and (B) analogously to our fittings of
Figures 5 and
6 : with a common set of
PNF-κB while each gene is fitted with its own
PG. Again, the average relative error is lower for up-regulated genes. (
D) Plots show single-gene traces (grey) of all mRNA (median: thick blue line; 85% and 15% intervals, thin blue lines). We show the median of the single-gene fits (thick red line) and the 85% and 15% intervals (thin red lines). (
E) Histograms on the bottom part show the distribution of the degradation rates obtained from the fitting for each dynamical cluster. Here, again we have that the degradation rate is the key parameter to reproduce the gene expression patterns observed.