Abstract
A spectrophotometric method for the estimation of N-acetyl-β-glucosaminidase in serum has been devised. Sera from normal adult males and females showed similar levels of activity.
The activity in serum rose progressively during pregnancy and fell rapidly after parturition to normal levels. This change resembled closely that which occurs in serum β-glucuronidase.
Placenta showed a moderate and chorion a high level of N-acetyl-β-glucosaminidase. High N-acetyl-β-glucosaminidase activity was demonstrated histochemically in decidual cells.
The functions of N-acetyl-β-glucosaminidase and β-glucuronidase and factors influencing their activity are discussed.
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- CONCHIE J., FINDLAY J. Influence of gonadectomy, sex hormones and other factors, on the activity of certain glycosidases in the rat and mouse. J Endocrinol. 1959 Mar;18(2):132–146. doi: 10.1677/joe.0.0180132. [DOI] [PubMed] [Google Scholar]
- CONCHIE J., FINDLAY J., LEVVY G. A. Alpha-mannosidase and other glycosidases in the tissues of the mouse and the rat, with special reference to the sex organs. Nature. 1956 Dec 29;178(4548):1469–1470. doi: 10.1038/1781469a0. [DOI] [PubMed] [Google Scholar]
- CONCHIE J., MANN T. Glycosidases in mammalian sperm and seminal plasma. Nature. 1957 Jun 8;179(4571):1190–1191. doi: 10.1038/1791190a0. [DOI] [PubMed] [Google Scholar]
- DUTTON G. J., STOREY I. D. Uridine compounds in glucuronic acid metabolism. I. The formation of glucuronides in liver suspensions. Biochem J. 1954 Jun;57(2):275–283. doi: 10.1042/bj0570275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARKNESS M. L., HARKNESS R. D. Changes in the physical properties and in the collagen and hexosamine contents of the foetal membranes during pregnancy in the rat. J Physiol. 1956 Jun 28;132(3):482–491. doi: 10.1113/jphysiol.1956.sp005541. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kerr L. M., Campbell J. G., Levvy G. A. beta-Glucuronidase as an index of growth in the uterus and other organs. Biochem J. 1949;44(4):487–494. doi: 10.1042/bj0440487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LINKER A., MEYER K., WEISSMANN B. Enzumatic formation of monosaccharides from hyaluronate. J Biol Chem. 1955 Mar;213(1):237–248. [PubMed] [Google Scholar]
- Neuberger A., Rivers R. V. The hydrolysis of glucosaminides by an enzyme in Helix pomatia. Biochem J. 1939 Oct;33(10):1580–1590. doi: 10.1042/bj0331580. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSEMAN S., DORFMAN A. alpha-Glucosaminidase. J Biol Chem. 1951 Aug;191(2):607–620. [PubMed] [Google Scholar]