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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1960 Nov;13(6):506–510. doi: 10.1136/jcp.13.6.506

THE DETERMINATION OF MAGNESIUM IN BIOLOGICAL FLUIDS AND TISSUES BY FLAME SPECTROPHOTOMETRY

Nancy Alcock 1, I MacIntyre 1, Ingeborg Radde 1
PMCID: PMC480131  PMID: 13682196

Abstract

A flame spectrophotometric method for estimating magnesium in biological fluids and tissues is described. It may be applied to the Zeiss spectrophotometer PMQ II with flame attachment. Interferences are small, and recoveries from plasma and urine and from ashed faeces, soft tissue, and bone are satisfactory. Calcium can be measured on the same dilutions of plasma and urine and faecal ash. Sodium, potassium, calcium, and magnesium can be estimated on the same dilutions of soft tissue and bone ash.

The plasma magnesium concentration of normal subjects falls within a narrow range. Differences between mean normal values previously reported may be partly attributed to the magnesium salt used in preparation of standard solutions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CARR M. H., FRANK H. A. Improved method for determination of calcium and magnesium in biologic fluids by EDTA titration. Am J Clin Pathol. 1956 Oct;26(10):1157–1168. doi: 10.1093/ajcp/26.10.1157. [DOI] [PubMed] [Google Scholar]
  2. DAVIS S. A flame photometric method for the determination of plasma magnesium after hydroxyquinoline precipitation. J Biol Chem. 1955 Oct;216(2):643–651. [PubMed] [Google Scholar]
  3. HUNTER G. Calcium and magnesium content of normal human blood serum. Nature. 1958 Jul 26;182(4630):263–264. doi: 10.1038/182263a0. [DOI] [PubMed] [Google Scholar]
  4. KAPUSCINSKI V., MOSS N., ZAK B., BOYLE A. J. Quantitative determination of calcium and magnesium in human serum by flame spectrophotometry. Am J Clin Pathol. 1952 Jul;22(7):687–691. doi: 10.1093/ajcp/22.7_ts.687. [DOI] [PubMed] [Google Scholar]
  5. MACINTYRE I., DAVIDSSON D. The production of secondary potassium depletion, sodium retention, nephrocalcinosis and hypercalcaemia by magnesium deficiency. Biochem J. 1958 Nov;70(3):456–462. doi: 10.1042/bj0700456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MACINTYRE I. The flame-spectrophotometric determination of calcium in biological fluids and an isotopic analysis of the errors in the Kramer-Tisdall procedure. Biochem J. 1957 Sep;67(1):164–172. doi: 10.1042/bj0670164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SCHACHTER D. The fluorometric estimation of magnesium in serum and in urine. J Lab Clin Med. 1959 Nov;54:763–768. [PubMed] [Google Scholar]
  8. TELOH H. A. Estimation of magnesium in serum by means of flame spectrophotometry. Am J Clin Pathol. 1958 Aug;30(2):129–132. doi: 10.1093/ajcp/30.2.129. [DOI] [PubMed] [Google Scholar]
  9. WACKER W. E., VALLEE B. L. A study of magnesium metabolism in acute renal failure employing a multichannel flame spectrometer. N Engl J Med. 1957 Dec 26;257(26):1254–1262. doi: 10.1056/NEJM195712262572602. [DOI] [PubMed] [Google Scholar]

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