Abstract
A new method for the determination of urinary formiminoglutamic acid (FIGLU) using conventional electrophoresis at 200 to 500 v. on cellulose acetate strips is reported. Experience in 166 determinations on 137 patients shows the method to be a simple, practical, and apparently sensitive one for the determination of FIGLU in the urine.
Results of the application of the measurement of urinary FIGLU with histidine loading as a test for folic acid deficiency are also reported.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAKERMAN H. A., SILVERMAN M., DAFT F. S. Influence of succinylsulfathiazole and folic acid on glutamic acid excretion. J Biol Chem. 1951 Jan;188(1):117–123. [PubMed] [Google Scholar]
- BOREK B. A., WAELSCH H. The enzymatic degradation of histidine. J Biol Chem. 1953 Nov;205(1):459–474. [PubMed] [Google Scholar]
- CHANARIN I., MOLLIN D. L., ANDERSON B. B. The clearance from the plasma of folic acid injected intravenously in normal subjects and patients with megaloblastic anaemia. Br J Haematol. 1958 Oct;4(4):435–446. doi: 10.1111/j.1365-2141.1958.tb06045.x. [DOI] [PubMed] [Google Scholar]
- HERBERT V., BAKER H., FRANK O., PASHER I., SOBOTKA H., WASSERMAN L. R. The measurement of folic acid activity in serum: a diagnostic aid in the differentiation of the megaloblastic anemias. Blood. 1960 Feb;15:228–235. [PubMed] [Google Scholar]
- HERBERT V., BAKER H., FRANK O., PASHER I., SOBOTKA H., WASSERMAN L. R. The measurement of folic acid activity in serum: a diagnostic aid in the differentiation of the megaloblastic anemias. Blood. 1960 Feb;15:228–235. [PubMed] [Google Scholar]
- HIATT H. H., GOLDSTEIN M., TABOR H. Urinary excretion of formiminoglutamic acid by human subjects after antifolic acid therapy. J Clin Invest. 1958 Jun;37(6):829–832. doi: 10.1172/JCI103671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAGAN G. A single-dose anthelmintic in general practice. Lancet. 1961 Jan 14;1(7168):112–112. doi: 10.1016/s0140-6736(61)92159-6. [DOI] [PubMed] [Google Scholar]
- KNOWLES J. P., PRANKERD T. A., WESTALL R. G. Simplified method for detecting formiminoglutamic acid in urine as a test of folic-acid deficiency. Lancet. 1960 Aug 13;2(7146):347–348. doi: 10.1016/s0140-6736(60)91486-0. [DOI] [PubMed] [Google Scholar]
- LUHBY A. L., COOPERMAN J. M., TELLER D. N. Urinary excretion of formiminoglutamic acid: application in diagnosis of clinical folic acid deficiency. Am J Clin Nutr. 1959 Jul-Aug;7:397–406. doi: 10.1093/ajcn/7.4.397. [DOI] [PubMed] [Google Scholar]
- SILVERMAN M., GARDINER R. C., BAKERMAN H. A. The nature of the glutamic acid excreted in folic acid deficiency. J Biol Chem. 1952 Feb;194(2):815–821. [PubMed] [Google Scholar]
- SPRAY G. H., WITTS L. J. Excretion of formiminoglutamic acid as an index of folic-acid deficiency. Lancet. 1959 Oct 31;2(7105):702–704. doi: 10.1016/s0140-6736(59)92132-4. [DOI] [PubMed] [Google Scholar]
- TABOR H., MEHLER A. H. Isolation of N-formyl-L-glutamic acid as an intermediate in the enzymatic degradation of L-histidine. J Biol Chem. 1954 Oct;210(2):559–568. [PubMed] [Google Scholar]
- TABOR H., WYNGARDEN L. A method for the determination of formiminoglutamic acid in urine. J Clin Invest. 1958 Jun;37(6):824–828. doi: 10.1172/JCI103670. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATERS A. H., MOLLIN D. L. Studies on the folic acid activity of human serum. J Clin Pathol. 1961 Jul;14:335–344. doi: 10.1136/jcp.14.4.335. [DOI] [PMC free article] [PubMed] [Google Scholar]