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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1961 Nov;14(6):654–660. doi: 10.1136/jcp.14.6.654

A stable sheep cell preparation for detecting thyroglobulin auto-antibodies and its clinical applications

A J Fulthorpe 1,2,3, I M Roitt 1,2,3, D Doniach 1,2,3, K Couchman 1,2,3
PMCID: PMC480310  PMID: 13895879

Abstract

The preparation of stable formolized sheep cells coated with thyroglobulin and their use for the detection of auto-antibodies in human thyroiditis is described. This provides an easy laboratory test which gives results closely comparable with those using fresh tanned cells.

The agglutination titres do not closely parallel the antibody content of the serum but a good correlation may be obtained by inhibiting agglutination with thyroglobulin.

The diagnostic usefulness of the test in treating disorders of the thyroid is discussed and related to the results of tests for the other two immune systems involved in thyroiditis. The tanned red cell test may be used to differentiate Hashimoto's disease from thyroid cancer and non-toxic colloid goitre, and to assess the choice of treatment in patients with Graves' disease. It has proved of particular value in genetic studies of auto-immunity.

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Selected References

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  1. ANDERSON J. R., GOUDIE R. B., GRAY K. G. The thyrotoxic complement-fixation reaction. Scott Med J. 1959 Feb;4(2):64–74. doi: 10.1177/003693305900400202. [DOI] [PubMed] [Google Scholar]
  2. BALFOUR B. M., DONLACH D., ROITT I. M., COUCHMAN K. G. Fluorescent antibody studies in human thyroiditis: auto-antibodies to an antigen of the thyroid colloid distinct from thyroglobulin. Br J Exp Pathol. 1961 Aug;42:307–316. [PMC free article] [PubMed] [Google Scholar]
  3. BELYAVIN G., TROTTER W. R. Investigations of thyroid antigens reacting with Hashimoto sera; evidence for an antigen other than thyroglobulin. Lancet. 1959 Mar 28;1(7074):648–652. doi: 10.1016/s0140-6736(59)92401-8. [DOI] [PubMed] [Google Scholar]
  4. BLIZZARD R. M., HAMWI G. J., SKILLMAN T. G., WHEELER W. E. Thyroglobulin antibodies in multiple thyroid diseases. N Engl J Med. 1959 Jan 15;260(3):112–116. doi: 10.1056/NEJM195901152600302. [DOI] [PubMed] [Google Scholar]
  5. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CLINE M. J., SELENKOW H. A., BROOKE M. S. Autoimmunity in thyroid disease. N Engl J Med. 1959 Jan 15;260(3):117–121. doi: 10.1056/NEJM195901152600303. [DOI] [PubMed] [Google Scholar]
  7. DONIACH D., HUDSON R. V., ROITT I. M. Human auto-immune thyroiditis: clinical studies. Br Med J. 1960 Feb 6;1(5170):365–373. doi: 10.1136/bmj.1.5170.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FULTHORPE A. J. Tetanus antitoxin titration by haemagglutination. J Hyg (Lond) 1957 Sep;55(3):382–401. doi: 10.1017/s0022172400037293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HACKETT E., BEECH M., FORBES I. J. Thyrogloubulin antibodies in patients without clinical disease of the thyroid gland. Lancet. 1960 Aug 20;2(7147):402–404. doi: 10.1016/s0140-6736(60)92842-7. [DOI] [PubMed] [Google Scholar]
  10. HALES I. B., MYHILL J., RUNDLE F. F., MACKAY I. R., PERRY B. Relation of eye signs in Graves' disease to circulating antibodies to thyroglobulin. Lancet. 1961 Mar 4;1(7175):468–469. doi: 10.1016/s0140-6736(61)90056-3. [DOI] [PubMed] [Google Scholar]
  11. HILL O. W. Thyroglobulin antibodies in 1,297 patients without thyroid disease. Br Med J. 1961 Jun 24;1(5242):1793–1796. doi: 10.1136/bmj.1.5242.1793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MACKAY I. R., PERRY B. T. Autoimmunity in human thyroid disease. Australas Ann Med. 1960 May;9:84–92. doi: 10.1111/imj.1960.9.2.84. [DOI] [PubMed] [Google Scholar]
  13. OWEN S. G., SMART G. A. Thyroid antibodies in myxoedema. Lancet. 1958 Nov 15;2(7055):1034–1036. doi: 10.1016/s0140-6736(58)90531-2. [DOI] [PubMed] [Google Scholar]
  14. PAINE J. R., TERPLAN K., ROSE N. R., WITEBSKY E., EGAN R. W. A clinical study of chronic noninfectious thyroiditis and autoimmunization. Surgery. 1957 Nov;42(5):799–813. [PubMed] [Google Scholar]
  15. ROITT I. M., DONIACH D. Human autoimmune thyroiditis: serological studies. Lancet. 1958 Nov 15;2(7055):1027–1033. doi: 10.1016/s0140-6736(58)90530-0. [DOI] [PubMed] [Google Scholar]
  16. SMART G. A., OWEN S. G. Thyroid antibodies and thyroiditis. Postgrad Med J. 1960 Jul;36:442–445. doi: 10.1136/pgmj.36.417.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TROTTER W. R., BELYAVIN G., WADDAMS A. Precipitating and complement-fixing antibodies in Hashimoto's disease. Proc R Soc Med. 1957 Nov;50(11):961–962. [PubMed] [Google Scholar]
  18. WITEBSKY E., ROSE N. R. Studies on organ specificity. IV. Production of rabbit thyroid antibodies in the rabbit. J Immunol. 1956 Jun;76(6):408–416. [PubMed] [Google Scholar]
  19. WITEBSKY E., ROSE N. R., TERPLAN K., PAINE J. R., EGAN R. W. Chronic thyroiditis and autoimmunization. J Am Med Assoc. 1957 Jul 27;164(13):1439–1447. doi: 10.1001/jama.1957.02980130015004. [DOI] [PubMed] [Google Scholar]

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