Abstract
A method for the determination of total cholesterol in serum is described. Optimum conditions are found for the isolation of the steroid free from extraneous chromogens, and a new sensitive colour reagent is introduced. The procedure is convenient for routine application, the final colour being light-stable and reasonably time-stable at room temperature. Comparisons are made with one other method and with another colour reagent. It is concluded that, for accurate results, treatment of the serum with alcoholic alkali followed by extraction with a hydrophobic solvent is essential. The choice of the colour-developing reagent is then no longer dependent on specificity for cholesterol, but becomes inter alia a matter of ease of operation, and of sensitivity, stability, and reproducibility of the final colour. These points are discussed in relation to other methods.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ABEL L. L., LEVY B. B., BRODIE B. B., KENDALL F. E. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem. 1952 Mar;195(1):357–366. [PubMed] [Google Scholar]
- ANDERSON J. T., KEYS A. Cholesterol in serum and lipoprotein fractions; its measurement and stability. Clin Chem. 1956 Jun;2(3):145–159. [PubMed] [Google Scholar]
- CONNERTY H. V., BRIGGS A. R., EATON E. H., Jr Simplified determination of the lipid components of blood serum. Clin Chem. 1961 Feb;7:37–53. [PubMed] [Google Scholar]
- MORRIS T. G. A comparison of methods for the estimation of serum cholesterol and values in random samples of populations in the 55-64 age group. J Clin Pathol. 1959 Nov;12:518–523. doi: 10.1136/jcp.12.6.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SPERRY W. M., WEBB M. A revision of the Schoenheimer-Sperry method for cholesterol determination. J Biol Chem. 1950 Nov;187(1):97–106. [PubMed] [Google Scholar]
- ZLATKIS A., ZAK B., BOYLE A. J. A new method for the direct determination of serum cholesterol. J Lab Clin Med. 1953 Mar;41(3):486–492. [PubMed] [Google Scholar]