Abstract
The Fe59 method of Suit (1957) has been used to estimate total bone marrow cellularity in 10 haematologically normal patients. A mean figure of 10·4 × 109 nucleated cells/kg. body weight was obtained. Simultaneous estimates from marrow obtained by rib excision produced a mean value of 11·1 × 109 nucleated cells/kg.
Previous work by this and other methods is reviewed. While some results agree well with those of the present work, one is higher by 50% and another by 300%. Corrections are suggested which reduce these figures and bring all published results within a small range.
Reasonably accurate determinations of total marrow cellularity in disease will not be possible until more is known of marrow Fe59 uptake in abnormal states.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOTHWELL T. H., CALLENDER S., MALLETT B., WITTS L. J. The study of erythropoiesis using tracer quantities of radioactive iron. Br J Haematol. 1956 Jan;2(1):1–16. doi: 10.1111/j.1365-2141.1956.tb06678.x. [DOI] [PubMed] [Google Scholar]
- BOTHWELL T. H., HURTADO A. V., DONOHUE D. M., FINCH C. A. Erythrokinetics. IV. The plasma iron turnover as a measure of erythropoiesis. Blood. 1957 May;12(5):409–427. [PubMed] [Google Scholar]
- DONOHUE D. M., GABRIO B. W., FINCH C. A. Quantitative measurement of hematopoietic cells of the marrow. J Clin Invest. 1958 Nov;37(11):1564–1570. doi: 10.1172/JCI103749. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ELLIS R. E. The distribution of active bone marrow in the adult. Phys Med Biol. 1961 Jan;5:255–258. doi: 10.1088/0031-9155/5/3/302. [DOI] [PubMed] [Google Scholar]
- OSGOOD E. E. Number and distribution of human hemic cells. Blood. 1954 Dec;9(12):1141–1154. [PubMed] [Google Scholar]
- PATT H. M. A consideration of myeloid-erythroid balance in man. Blood. 1957 Sep;12(9):777–787. [PubMed] [Google Scholar]
- PEGG D. E., KEMP N. H. Collection, storage, and administration of autologous human bone-marrow. Lancet. 1960 Dec 31;2(7166):1426–1429. doi: 10.1016/s0140-6736(60)92571-x. [DOI] [PubMed] [Google Scholar]
- REIFF R. H., NUTTER J. Y., DONOHUE D. M., FINCH C. A. The relative number of marrow reticulocytes. Am J Clin Pathol. 1958 Sep;30(3):199–203. doi: 10.1093/ajcp/30.3.199. [DOI] [PubMed] [Google Scholar]
- SUIT H. D. A technique for estimating the bone marrow cellularity in vivo using 59Fe. J Clin Pathol. 1957 Aug;10(3):267–269. doi: 10.1136/jcp.10.3.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZILVA J. F., NICHOLSON J. P. An obesity index and the significance of bromide space measurements. Clin Sci. 1960 Aug;19:449–456. [PubMed] [Google Scholar]