Fig. 1.

Amplification of VDR gene and restriction endonuclease digestion pattern for FokI, BsmI, ApaI and TaqI polymorphims. The 265 bp fragment (a) was digested by FokI enzyme (b) generating two fragments, 196 (2) and 69 bp (3) for T allele. C allele, containing 265 bp (1), represents the absence of restriction site. The 825 bp fragment (c) was digested by BsmI enzyme (d) presenting two fragments, 650 (2) and 175 bp (3) for G allele, and 825 bp (1) for A allele (no restriction site). The amplicon of 325 bp (e) was used for detection of ApaI and TaqI polymorphims. For ApaI, the 352 bp fragment (1) represents T allele, and the 214 bp (2) and 18 bp (3) fragments indicate the presence of G allele (f). Finally, after digestion with TaqI enzyme (g) it was verified two fragments, 293 (2) and 59 bp (3) for C allele and a unique fragment of 352 bp (1) for T allele. M: 100 bp DNA ladder. Lanes 1–4: represent PCa patients; Lanes 5–8: represent BPH patients; Lanes 9–12: represent healthy volunteers. NC negative control