Abstract
The capacity of blood platelets to attach themselves aspecifically to antigen-antibody complexes in the presence of complement can be utilized for the demonstration of antibodies formed against homologous tissues.
A mixture of the serum-, antigen-complement, and of human thrombocytes is incubated, and the number of thrombocytes in the supernatant is ascertained and referred to a standard. Measurements less than 85% of the standard are regarded as positive.
Sera collected from patients with `protracted' acute or chronic hepatitis and cirrhosis were found to be positive with the new method in 68·0% of the cases; the corresponding figures were 26·3% for `non-protracted' acute hepatitis and 18·1% for diseases of the bile duct. No positive reaction occurred in other internal diseases studied or in healthy persons, but 40% of subjects with diverse infections gave positive results possibly because of autoantibody formation against tissues damaged by the infections. Comparative examinations suggest that the thrombocyte consumption test is more specific than the antihuman globulin consumption method.
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- HOIGNE R., STORCK H. Uber die Bedeutung der Thrombocyten bei allergischen Vorgängen. II. Thrombocytenagglutination in Patientenblut nach Zugabe von Allergen. Schweiz Med Wochenschr. 1953 Aug 1;83(31):718–721. [PubMed] [Google Scholar]
- KOROSSY S., GOZONY M. [Studies with staphylococcal anatoxin in coccogenic eczematous patients]. Acta Allergol. 1959;13:286–294. [PubMed] [Google Scholar]
- LAMANNA C. Adhesion of foreign particles to particulate antigens in the presence of antibody and complement (serological adhesion). Bacteriol Rev. 1957 Mar;21(1):30–45. doi: 10.1128/br.21.1.30-45.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NELSON R. A., Jr, NELSON D. S. On the mechanism of immune-adherence. II. Analogy to mixed aggregation of sensitized antigens in the presence of complement; immune-adherence with animal platelets. Yale J Biol Med. 1959 Feb;31(4):201–212. [PMC free article] [PubMed] [Google Scholar]
- OSLER A. G., HAWRISIAK M. M., OVARY Z., SIQUEIRA M., BIER O. G. Studies on the mechanism of hypersensitivity phenomena. II. The participation of complement in passive cutaneous anaphylaxis of the albino rat. J Exp Med. 1957 Dec 1;106(6):811–834. doi: 10.1084/jem.106.6.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OSZTOVICS M., MARCSEK Z., SZASZ G. [Data on immunopathological aspects of rheumatic fever in children. I. Experience with the anti-globulin consumption test]. Gyermekgyogyaszat. 1962 Apr;13:97–106. [PubMed] [Google Scholar]
- RICE C. E. An investigation of some of the factors determining the decrease in complement activity in anaphylactic shock. J Immunol. 1955 Aug;75(2):85–95. [PubMed] [Google Scholar]
- RISSEL E., STEFFEN C., WEWALKA F. Serumfaktoren mit Gewebsantikörpereigenschaften bei Lebererkrankungen. Wien Klin Wochenschr. 1957 Nov 22;69(47):885–890. [PubMed] [Google Scholar]
- ROCHA e SILVA M. The role played by leucocytes and platelets in anaphylactic and peptone shock. Ann N Y Acad Sci. 1950 Apr 28;50(9):1045–1067. doi: 10.1111/j.1749-6632.1950.tb39902.x. [DOI] [PubMed] [Google Scholar]
- STAVITSKY A. B., STAVITSKY R., ECKER E. E. Loss of hemolytic-complement activity and of granulocytes following reinjection of an antigen into the rabbit. J Immunol. 1949 Dec;63(4):389–407. [PubMed] [Google Scholar]
- STEFFEN C., SCHINDLER H. Untersuchungen über die Eigenschaften einer im Serum von Polyarthritikern und von Patienten mit rheumatischer Endocarditis vorkommenden Substanz mit den Merkmalen eines gewebsspezifischen Antikörpers. Schweiz Z Pathol Bakteriol. 1955;18(3):287–302. [PubMed] [Google Scholar]
- STEFFEN C. Untersuchung über das Vorkommen eines in Polyarthritikerseren und in Seren von Endokarditiskranken aufscheinenden und an Bindegewebszellen sessil werdenden Antikörpers. Wien Z Inn Med. 1954 Oct;35(10):422–429. [PubMed] [Google Scholar]
- STORCK H., HOIGNE R., KOLLER F. Zur Pathogenese der Purpura. Schweiz Med Wochenschr. 1951 Feb 24;81(8):195–196. [PubMed] [Google Scholar]
- STORCK H., HOIGNE R. Thrombozyten und Serologie bei medikamentösen Hautallergien. Dermatologica. 1956 Apr-Jun;112(4-6):405–418. [PubMed] [Google Scholar]