. Author manuscript; available in PMC: 2016 Mar 24.

Published in final edited form as: Biochemistry. 2016 Mar 3;55(10):1485–1493. doi: 10.1021/acs.biochem.5b01364

Table 1. Example Fit Relaxation Rates and Amplitudes for Temperature-Jump Data.

	DHFR·folate^a	DHFR·NADP⁺^a	DHFR·NADP⁺·folate^b	DHFR apoenzyme
slow rate (s⁻¹)	400 ± 70	300 ± 100	400 ± 100	240 ± 40
slow rate amplitude	2.7 ± 0.3	1.2 ± 0.2	2.4 ± 0.2	2.3 ± 0.5
fast rate (s⁻¹)	3800 ± 200	2500 ± 91	2200 ± 500	5000 ± 1000
fast rate amplitude	4.9 ± 0.5	2.0 ± 0.2	1.8 ± 0.5	0.6 ± 0.08

Reported values are the average of the data taken at 100 μM DHFR and 150 μM ligand.

Reported values are the average of the data taken at 100 μM DHFR, 1000 μM NADP⁺, and 150 μM folate.