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. 1993 Dec 15;90(24):11777–11781. doi: 10.1073/pnas.90.24.11777

Evolution and expression of a beta-like globin gene of the Australian marsupial Sminthopsis crassicaudata.

S J Cooper 1, R M Hope 1
PMCID: PMC48067  PMID: 8265626

Abstract

A beta-like globin gene was isolated from the Australian dasyurid marsupial Sminthopsis crassicaudata. Nucleotide-sequence analysis of promoter and coding regions of the gene revealed that it was orthologous to eutherian early-expressed (epsilon, gamma, eta) beta-like globin genes. Comparison of the conceptually translated sequence of the gene with a partial amino acid sequence of the adult beta-globin chain from S. crassicaudata provided evidence that the gene was not expressed in adult tissues. In addition, Northern analysis of RNA isolated from an embryo, pouch young, and adult bone marrow indicated that the gene was expressed predominantly in embryonic tissues and that there was a significant reduction in the expression of the gene within a day of birth. These results provide strong support for the hypothesis of Koop and Goodman [Koop, B. F. & Goodman, M. (1988) Proc. Natl. Acad. Sci. USA 85, 3893-3897] that an embryonic beta-like globin gene existed prior to the divergence of the eutherian and marsupial lineages and that this gene was already differentiated with respect to its promoter regions and developmental expression. The observation that epsilon-globin mRNA was present at least until day 4 postpartum suggests that the epsilon-globin chain may play some role in influencing the physiological properties of hemoglobin in S. crassicaudata neonates.

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Selected References

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  1. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  2. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  3. Clegg J. B., Naughton M. A., Weatherball D. J. Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok). J Mol Biol. 1966 Aug;19(1):91–108. doi: 10.1016/s0022-2836(66)80052-9. [DOI] [PubMed] [Google Scholar]
  4. Efstratiadis A., Posakony J. W., Maniatis T., Lawn R. M., O'Connell C., Spritz R. A., DeRiel J. K., Forget B. G., Weissman S. M., Slightom J. L. The structure and evolution of the human beta-globin gene family. Cell. 1980 Oct;21(3):653–668. doi: 10.1016/0092-8674(80)90429-8. [DOI] [PubMed] [Google Scholar]
  5. Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
  6. Goodman M., Koop B. F., Czelusniak J., Weiss M. L. The eta-globin gene. Its long evolutionary history in the beta-globin gene family of mammals. J Mol Biol. 1984 Dec 25;180(4):803–823. doi: 10.1016/0022-2836(84)90258-4. [DOI] [PubMed] [Google Scholar]
  7. Hardison R. C. Comparison of the beta-like globin gene families of rabbits and humans indicates that the gene cluster 5'-epsilon-gamma-delta-beta-3' predates the mammalian radiation. Mol Biol Evol. 1984 Sep;1(5):390–410. doi: 10.1093/oxfordjournals.molbev.a040326. [DOI] [PubMed] [Google Scholar]
  8. Harris S., Barrie P. A., Weiss M. L., Jeffreys A. J. The primate psi beta 1 gene. An ancient beta-globin pseudogene. J Mol Biol. 1984 Dec 25;180(4):785–801. doi: 10.1016/0022-2836(84)90257-2. [DOI] [PubMed] [Google Scholar]
  9. Holland R. A., Rimes A. F., Comis A., Tyndale-Biscoe C. H. Oxygen carriage and carbonic anhydrase activity in the blood of a marsupial, the Tammar wallaby (Macropus eugenii), during early development. Respir Physiol. 1988 Jul;73(1):69–86. doi: 10.1016/0034-5687(88)90128-4. [DOI] [PubMed] [Google Scholar]
  10. Hope R., Bennett H., Chesson C., Cooper S. Restriction fragment length variants in the marsupial Sminthopsis crassicaudata. Biochem Genet. 1992 Aug;30(7-8):361–370. doi: 10.1007/BF00569327. [DOI] [PubMed] [Google Scholar]
  11. Koop B. F., Goodman M. Evolutionary and developmental aspects of two hemoglobin beta-chain genes (epsilon M and beta M) of opossum. Proc Natl Acad Sci U S A. 1988 Jun;85(11):3893–3897. doi: 10.1073/pnas.85.11.3893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Li W. H., Wu C. I., Luo C. C. A new method for estimating synonymous and nonsynonymous rates of nucleotide substitution considering the relative likelihood of nucleotide and codon changes. Mol Biol Evol. 1985 Mar;2(2):150–174. doi: 10.1093/oxfordjournals.molbev.a040343. [DOI] [PubMed] [Google Scholar]
  13. Richardson B. J., Russell E. M. Changes with ages in the proportion of nucleated red blood cell types and in the type of haemoglobin in kangaroo pouch young. Aust J Exp Biol Med Sci. 1969 Oct;47(5):573–580. doi: 10.1038/icb.1969.151. [DOI] [PubMed] [Google Scholar]
  14. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  15. Shapiro S. G., Schon E. A., Townes T. M., Lingrel J. B. Sequence and linkage of the goat epsilon I and epsilon II beta-globin genes. J Mol Biol. 1983 Sep 5;169(1):31–52. doi: 10.1016/s0022-2836(83)80174-0. [DOI] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Thompson E. O., Hosken R., Air G. M. Studies on marsupial proteins. I. Polymorphism of haemoglobin of the grey kangaroo, Macropus giganteus. Aust J Biol Sci. 1969 Apr;22(2):449–462. doi: 10.1071/bi9690449. [DOI] [PubMed] [Google Scholar]
  18. Tibben E. A., Holland R. A., Tyndale-Biscoe C. H. Blood oxygen carriage in the marsupial, tammar wallaby (Macropus eugenii), at prenatal and neonatal stages. Respir Physiol. 1991 Apr;84(1):93–104. doi: 10.1016/0034-5687(91)90021-a. [DOI] [PubMed] [Google Scholar]
  19. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wainwright B., Hope R. Cloning and chromosomal location of the alpha- and beta-globin genes from a marsupial. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8105–8108. doi: 10.1073/pnas.82.23.8105. [DOI] [PMC free article] [PubMed] [Google Scholar]

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