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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1964 Mar;17(2):163–164. doi: 10.1136/jcp.17.2.163

An improved method for the simple and accurate colorimetric determination of urea with Ehrlich's reagent

H Yatzidis 1,2, M Garidi 1,2, C Vassilikos 1,2, D Mayopoulou 1,2, A Akilas 1,2
PMCID: PMC480710  PMID: 14149943

Abstract

An improved method for the colorimetric determination of urea is described. The method is simple, requiring a deproteinized filtrate obtained after treatment of a small quantity of whole oxalated blood, serum, plasma or urine with 12·5% trichloracetic acid containing 50 g. activated carbon. The addition of Ehrlich's reagent to this filtrate results in the immediate production of a stable yellow colour. The determination is accurate, very sensitive and rapid, taking place in only 10 minutes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. LEVINE J. M., LEON R., STEIGMANN F. A rapid method for the determination of urea in blood and urine. Clin Chem. 1961 Oct;7:488–493. [PubMed] [Google Scholar]

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