Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1964 May;17(3):225–230. doi: 10.1136/jcp.17.3.225

A comparison of a fluorescent antibody technique with a cultural method in the detection of infections with Shigella sonnei

C E D Taylor 1,2, G V Heimer 1,2, D J Lea 1,2, A J H Tomlinson 1,2
PMCID: PMC480729  PMID: 14159448

Abstract

A comparison has been made of a fluorescent antibody technique with a cultural method for the detection of Sh. sonnei in faeces. The two methods were in agreement in 73% of the 394 specimens examined; 57 (14.5%) speciments were positive by culture only; 52 (13.2%) specimens gave positive results by fluorescence microscopy which were not confirmed by culture. Most of the latter are thought to be `false positive' results.

The value and usefulness of fluorescence microscopy in the diagnosis of Sonne dysentery is discussed.

Full text

PDF
225

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIEGELEISEN J. Z., Jr, SCOTT L. V., LEWIS V., Jr Rapid diagnosis of herpes simplex virus infections with fluorescent antibody. Science. 1959 Mar 6;129(3349):640–641. doi: 10.1126/science.129.3349.640. [DOI] [PubMed] [Google Scholar]
  2. CARVER R. K., GOLDMAN M. Staining Toxoplasma gondii with fluorescein-labeled antibody. III. The reaction in frozen and paraffin sections. Am J Clin Pathol. 1959 Aug;32(2):159–164. doi: 10.1093/ajcp/32.2.159. [DOI] [PubMed] [Google Scholar]
  3. GOLDSTEIN G., SLIZYS I. S., CHASE M. W. Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins. J Exp Med. 1961 Jul 1;114:89–110. doi: 10.1084/jem.114.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GORDON M. A. Differentiation of yeasts by means of fluorescent antibody. Proc Soc Exp Biol Med. 1958 Mar;97(3):694–698. doi: 10.3181/00379727-97-23849. [DOI] [PubMed] [Google Scholar]
  5. LIU C. Rapid diagnosis of human influenza infection from nasal smears by means of fluorescein-labeled antibody. Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):883–887. doi: 10.3181/00379727-92-22642. [DOI] [PubMed] [Google Scholar]
  6. METZGER J. F., SMITH C. W. Rapid identification of Neisseria meningitidis by fluorescent antibody technic. U S Armed Forces Med J. 1960 Oct;11:1185–1189. [PubMed] [Google Scholar]
  7. MOODY M. D., WINTER C. C. Rapid identification of Pasteurella pestis with fluorescent antibody. III. Staining Pasteurella pestis in tissue impression smears. J Infect Dis. 1959 May-Jun;104(3):288–294. doi: 10.1093/infdis/104.3.288. [DOI] [PubMed] [Google Scholar]
  8. McENTEGART M. G., CHADWICK C. S., NAIRN R. C. Fluorescent antisera in the detection of serological varieties of Trichomonas vaginalis. Br J Vener Dis. 1958 Mar;34(1):1–3. doi: 10.1136/sti.34.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RINDERKNECHT H. A new technique for the fluorescent labelling of proteins. Experientia. 1960 Sep 15;16:430–431. doi: 10.1007/BF02178856. [DOI] [PubMed] [Google Scholar]
  10. RODRIGUEZ J., DEINHARDT F. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology. 1960 Oct;12:316–317. doi: 10.1016/0042-6822(60)90205-1. [DOI] [PubMed] [Google Scholar]
  11. SMITH C. W., METZGER J. F., HOGGAN M. D. Immunofluorescence as applied to pathology. Am J Clin Pathol. 1962 Jul;38:26–42. doi: 10.1093/ajcp/38.1.26. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES