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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Methods. 2015 Dec 17;98:26–33. doi: 10.1016/j.ymeth.2015.12.009

Figure 4. Imaging RNA in mammalian cells with MG, DFHBI and PFP-DFHBI.

Figure 4

A) Images of CHO cells transiently transfected with plasmids from which either 4xMGA or control RNA was expressed. The cells were imaged with MG 9 h after transfection, B) Time course of the increase in fluorescence background after the addition of 10 µM MG to the cell medium, C) Mv1Lu cells were transiently transfected with plasmids from which control or SPN1A RNA were expressed from the CMV promoter. All cells were cotransfected with a plasmid from which DsRed was expressed from the CMV promoter. DsRed was used as an internal control to verify transfection of the cells and transcription of RNA from the plasmids. Cells were imaged 24h after transfection.