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MALDI-MS/MS spectrum of a permethylated N-glycan chain bearing the non-sulfated HNK1-epitope at m/z 2430 (M + Na+), derived by PNGAseF-digestion of Nid1 coexpressed with dGlcAT-P in CHO-Lec2 cells. The glucuronylated glycan as well as part of the fragments were detected with a mass incremental loss of 32 Da, corresponding to the elimination of methanol. The presence of terminal GlcA is indicated by B series ions (B3α and B3α-32) and by double fragmentation ions (B3αY5α).
