Figure 4. Genetic ablation of Pax7 results mainly in UPS development.
(A) Schematic representation of the system used to generate Ckm Hgf Cdkn2a-null Pax7-null (MHI-null P-null) mice. (B) Kaplan-Meier curve showing the relative sarcoma-free survival in the indicated genotypes. (C) Penetrance (pie charts) and histological distribution (bar graphs) of tumors. (D) Representative immunohistochemical analysis of MHI-null tumors in Pax7 heterozygous or null genetic background. UPS: undifferentiated pleomorphic sarcoma. (Scale bar = 250 µm). (E) Western blot analysis of murine UPS and ERMS probed for the indicate proteins. (F) Sca1 and Int-α7 expression analysis in primary murine UPS and ERMS (left panel, single staining; center and right panels, double staining).