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. 2016 Mar 8;5:e12509. doi: 10.7554/eLife.12509

Figure 1. Chemical structures of different designs of Leiker.

The top panel shows four designs of two-piece Leiker with a photo-cleavage site (sulfo-PL, PL, and PEG-PL) or an azobenzene-based cleavage site (AL). Biotin is attached via click chemistry by reacting with bio-aizde. The bottom panel shows two unlabeled (bAL1, bAL2) and deuterium-labeled ([d6]-bAL2) one-piece Leiker molecules. The biotin moiety is colored magenta.

DOI: http://dx.doi.org/10.7554/eLife.12509.003

Figure 1.

Figure 1—figure supplement 1. Optimization of protein-to-cross-linker ratio (w/w) for (A) sulfo-PL, (B) AL, (C) bAL1, and (D) bAL2.

Figure 1—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.12509.004
Figure 1—figure supplement 2. Evaluation of azobenzene-based chemical cleavage.

Figure 1—figure supplement 2.

DOI: http://dx.doi.org/10.7554/eLife.12509.005
Figure 1—figure supplement 3. The one-piece Leiker (bAL1) outperformed the two-piece Leiker (AL) in the CXMS analysis of a mixture of ten standard proteins.

Figure 1—figure supplement 3.

DOI: http://dx.doi.org/10.7554/eLife.12509.006
Figure 1—figure supplement 4. Evaluation of the two piece Azo-Leiker (AL).

Figure 1—figure supplement 4.

DOI: http://dx.doi.org/10.7554/eLife.12509.007
Figure 1—figure supplement 5. bAL1 and bAL2 performed similarly.

Figure 1—figure supplement 5.

DOI: http://dx.doi.org/10.7554/eLife.12509.008
Figure 1—figure supplement 6. MS1 spectra of (A) [d0]-bAL2 and (B) [d6]-bAL2.

Figure 1—figure supplement 6.

DOI: http://dx.doi.org/10.7554/eLife.12509.009