Figure 3. Evaluating the performance of Leiker.
(A) Leiker allowed near 100% enrichment of target peptides from a cross-linked ten-protein mixture diluted with increasing amounts of non-cross-linked E. coli lysates. Dark blue, inter-links; light blue, mono-links; green, loop-links; grey, regular peptides not modified by Leiker. (B) Number of cross-link identifications from E. coli lysates treated with Leiker or BS3. Shown in the left and right panels are the identified spectra and peptides, respectively.
DOI: http://dx.doi.org/10.7554/eLife.12509.011
Figure 3—source data 1. Ten standard proteins used to evaluate Leiker, mixed at equal amounts by mass.
elife-12509-fig3-data1.xlsx (8.8KB, xlsx)
DOI: 10.7554/eLife.12509.012
Figure 3—source data 2. Summary of identified spectra from the ten-protein mixture.
elife-12509-fig3-data2.xlsx (55.1KB, xlsx)
DOI: 10.7554/eLife.12509.013
Figure 3—figure supplement 1. Distance distributions of cross-linked lysine pairs in the undiluted ten-protein mixture.
