Fig. 3.

Chinmo is not acting through the male determinant Dsx^M to masculinize the ovary. (A,B) Immunofluorescence detection in adult ovaries of FasIII (green at cell periphery) to visualize somatic cell membranes, and 1B1 (green in germ cells) to mark fusomes. Before ectopic expression of Dsx^M, ovaries look normal (A). After ectopic overexpression (OE) of Dsx^M in somatic cells in adult germaria (B), ovaries are also indistinguishable from wild-type ovaries. (C,D) Immunofluorescence detection of the male-specific protein Dsx^M (green). Dsx^M is not expressed in control ovaries (C) or in chinmo overexpression ovaries after five days of ectopic chinmo expression in adult somatic cells (D). Vasa (red) marks germ cells and DAPI (blue) marks nuclei in all panels. Scale bars: 20 μm.