Table 4. List of primer sequences used for qRT–PCR analysis with their annealing temperatures.
| Target | Sequence (5'–3') | Annealing temperature | |
|---|---|---|---|
| Kaiso | Forward | AGAGGAAAGGGCATGGAGAGT | 60.8 °C |
| Reverse | GGCCACGTTGCTCATTCAAG | ||
| TGFβR1 | Forward | CCGTTTGTATGTGCACCCTC | 60 °C |
| Reverse | GCCAGGTGATGACTTTACAGTAGT | ||
| TGFβR2 | Forward | CTCGGTCTATGACGAGCAGC | 60 °C |
| Reverse | CCTCCATTTCCACATCCGACT | ||
| ZEB-1 | Forward | AGAATTCACAGTGGAGAGAAGCC | 60 °C |
| Reverse | CGTTTCTTGCAGTTTGGGCATT | ||
| ANGPTL4 | Forward | CAGCCTGCAGACACAACTCA | 60 °C |
| Reverse | ATTCGCAGGTGCTGCTTCTC | ||
| β-Actin | Forward | CTCTTCCAGCCTTCCTTCCT | 55 °C |
| Reverse | AGCACTGTGTTGGCGTACAG | ||
Abbreviation: qRT–PCR, quantitative reverse transcription–PCR.