Abstract
Corynebacterium ulcerans is a pathogenic bacterium infecting wild and domesticated animals; some infection cases in humans have increased throughout the world. The current study describes the draft genome of strain 04-3911, isolated from humans. The draft genome has 2,492,680 bp, 2,143 coding sequences, 12 rRNA genes, and 50 tRNA genes.
GENOME ANNOUNCEMENT
Corynebacterium ulcerans belongs to a suprageneric group of actinomycetes that also includes the genera Mycobacterium, Nocardia, and Rhodococcus, termed the CMNR group (Corynebacterium, Mycobacterium, Nocardia, and Rhodococcus). This bacterium is facultative anaerobic, nonsporulating, nonmotile, catalase positive, and nitrate and oxidase negative (1, 2). Analyses of 16S rRNA gene sequences showed that C. ulcerans, Corynebacterium pseudotuberculosis, and Corynebacterium diphtheriae are closely related (2). Otherwise, there are C. ulcerans strains coding for a diphtheria toxin similar to that encoded by toxigenic strains of Corynebacterium diphtheriae (3), as well as C. ulcerans strains coding for a dermonecrotic toxin with similarity to toxic phospholipase D (PLD) from C. pseudotuberculosis (4).
C. ulcerans has been detected in a variety of wild and domesticated animals, suggesting that both groups may attend as reservoirs for zoonotic transmission (5). This bacterium is an emergent pathogen due its frequency and severity of human infections reported during the last two decades in various countries (6). In humans, it causes diphtheria-like disease, pharyngitis, sinusitis, tonsillitis, pulmonary nodules, and skin ulcers (7). Here, we present the draft genome sequence of C. ulcerans 04-3911, isolated from humans. The genome sequencing was performed by the SOLiD platform, using a fragment library. The predicted genome coverage was approximately 6,000×, based on C. ulcerans genomes available in GenBank (http://www.ncbi.nlm.nih.gov/genbank/). The software Velvet version 1.2.10 (8) was used with a de novo-assembled strategy. The assembly generated 40 contigs, with 2,492,680 bp, which were submitted to GenBank to automatic annotation. The genome has 2,143 coding sequences, 12 rRNA genes, 50 tRNA genes, 55 pseudogenes, 2 clustered regularly interspaced short palindromic repeat (CRISPR) arrays, and a G+C content of 53.30%. This genome is part of further studies of comparative genomics, pathogenicity, and vaccine and drug targets of the species.
Nucleotide sequence accession numbers.
The C. ulcerans whole-genome shotgun (WGS) project has the project accession no. LGSX00000000. The version described in this paper is version LGSX01000000 and consists of sequences LGSX01000001 to LGSX01000040.
ACKNOWLEDGMENTS
This work was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES) and National Council for Scientific and Technological Development (CNPq) and the Genome and Proteome Network of the State of Pará (RPGP).
Footnotes
Citation Guimarães LC, Viana MVC, Benevides LJ, Mariano DCB, Veras AAO, Sá PHC, Rocha FS, Vilas Boas PCB, Soares SC, Barbosa MS, Guiso N, Badell E, Carneiro AR, Azevedo V, Ramos RTJ, Silva A. 2016. Draft genome sequence of Corynebacterium ulcerans strain 04-3911, isolated from humans. Genome Announc 4(2):e00171-16. doi:10.1128/genomeA.00171-16.
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