Figure 6.
E2 and BPA decreased expression of Icam5 in vitro. Pituitaries treated in vitro with E2 (10−8M) showed decreased expression of Icam5, this decrease was inhibited by cotreatment of E2+ICI in males (A) and females (B) (n = 6–9). Pituitaries treated with BPA (4.4 × 10−6M) demonstrated decreased expression of Icam5, which was blocked fully by cotreatment of BPA+ICI in males (C) but only partially blocked the decrease in females (BPA+ICI significantly lower than control in females) (D). ICI alone had no effect in males (C) or females (D) (n = 6–9). A dose-response curve showed that BPA decreased expression of Icam5 at the 10−6M and 10−5M concentrations but had no effect at lower concentrations in vitro (n = 4–5) (E). *, P = .05.