ATP‐driven Rad50 conformations regulate DNA tethering, end resection, and ATM checkpoint signaling

Abstract

The methods currently state on p. 498 that 1,000 units E. coli DNA ligase (NAD dependent, Fig 5) or 10,000 units T4 DNA ligase (Fig 7) were used, but these values are the concentrations of the enzyme preparations in units per ml, not the amounts that were used in the reactions. The actual units that were used in the 10 μl reactions were 1 unit of E. coli DNA ligase and 40 units of T4 DNA ligase.