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. 1992 Jan 1;89(1):241–245. doi: 10.1073/pnas.89.1.241

Patchy accumulation of apical Na+ transporters allows cross talk between extracellular space and cell nucleus.

H Oberleithner 1, S Wünsch 1, S Schneider 1
PMCID: PMC48212  PMID: 1309607

Abstract

Intracellular Na+ activities and local current densities were measured in fused Madin-Darby canine kidney cells using Na+ and voltage-sensing microelectrodes. Na+ that enters the cell across the apical plasma membrane accumulates initially in the nucleoplasm, several seconds ahead of its appearance in the cell cytoplasm. The spatial distribution of Na+ currents, produced by a local superfusion of the cell surface, indicates a nonuniform, patchy accumulation of apical Na+ transporters in the vicinity of the nucleus. Such pathways for direct Na+ flux between extracellular space and cell nucleus could be potentially important for gene activation.

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Selected References

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