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. Author manuscript; available in PMC: 2016 Jul 8.
Published in final edited form as: Oncogene. 2015 Oct 5;35(22):2913–2922. doi: 10.1038/onc.2015.348

Figure 2. Quinacrine uniquely promotes apoptosis in lung tumors in vivo.

Figure 2

(A) Immunostaining for autophagosome accumulation (punctate LC3) and p62 (SQSTM1) aggregates in A549 xenograft tumors treated with CQ and Q. Bar, 50μm. (B) Increased areas of necrosis and dying cells in Q-treated tumors. Upper panel bar, 200μm; lower panel bar, 50μm. (C) Immunostaining for phospho-histone H3 (pHH3, mitosis marker) and cleaved caspase-3 (CC3, apoptosis marker) of lung tumors treated with CQ and Q. Bar, 100μm. (D, E) Quantification of pHH3 (D) and CC3 (E) positive tumor cells. Bar, 100μm. Data represent mean ± SEM from 6 independent tumors for each cohort. Statistical significance was calculated using ANOVA followed by Tukey’s HSD. ***P≤0.001.