Table 1.
Incubation time
|
Conversionβ
|
|
---|---|---|
Leu-enkephalin-Arg6 | dynorphin A(14–17) | |
4 h | 1.9% | 0.72% |
72 h | 56.5% | 3.2% |
100 μM dynorphin A was added into the medium of striatal neuron cultures at 7 days in vitro and analyzed after 4 h or 72 h. The background peptide levels were at least 1000 fold lower in cell culture medium incubated without dynorphin A. No Leu-enkephalin-Arg6 and dynorphin A (14–17) were detected in the medium immediately after dynorphin A was added.
Total amount of dynorphin A plus Leu-enkephalin-Arg6 or dynorphin A plus dynorphin A (14–17), was taken as 100%.