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. 2015 Dec 2;7(3):3283–3296. doi: 10.18632/oncotarget.6488

Figure 6. REIC/DKK-3 is a part of the AR complex including SGTA and TCTEX-1.

Figure 6

A. The pull-down (PD) assays of AR complex. Halo-tagged REIC/DKK-3, HA-tagged SGTA, FLAG-tagged TCTEX-1 and GFP-tagged AR were expressed in PC3 cell. Halo tag pull-down was performed. For the PD assay of the investigation of the component including AR complexes, Halo-tagged REIC/DKK-3, HA-tagged SGTA, FLAG-tagged TCTEX-1 and GFP-tagged AR were expressed in PC3 cells. Cell lysates from both plasmids transfected PC3 cells were analyzed. The sample pulled down by Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-Halo, -HA, -FLAG and -GFP and antibodies B. The effects of REIC/DKK-3 and TCTEX-1 expression on AR activity were examined in PC3 cells. Under co-transfection with AR, REIC/DKK-3 and/or TCTEX-1 plasmids, the cells were transfected with the p159-pPr-Luc plasmid vector and were treated with DHT. The value for the baseline was obtained using the vehicle control. The results are derived from three independent experiments. *, A significant difference was observed.