Abstract
Klebsiella pneumoniae sequence type (ST) 307, carrying blaKPC-3, blaCTX-M-15, blaOXA-1, aac(6′)-Ib-cr, and qnrB1 genes, is replacing the predominant hyperepidemic ST258 clone in Italy. Whole-genome and complete plasmid sequencing of one ST307 strain was performed and new features were identified.
GENOME ANNOUNCEMENT
KPC carbapenemase-producing Klebsiella pneumoniae of sequence type (ST) 258 and related variants (Clonal Group CG258) emerged during the early 2000s in the United States and have spread throughout the world (1). A countrywide survey on carbapenem nonsusceptible K. pneumoniae isolates showed that the most frequent clones circulating in Italian hospitals belonged to CG258 (2). Specific IncF plasmids with blaKPC (pKpQIL) and virulence traits (pKPN3) have contributed significantly to the success of CG258 (3, 4). In more recent surveillance studies from South Italy, multifocal dissemination of KPC-3-producing K. pneumoniae (KPC-3-Kp) clones was observed, showing the rapid emergence of the KPC-3-Kp ST307 clone, also coproducing the CTX-M-15 extended spectrum beta-lactamase (ESBL). Here, we report the whole-genome sequencing (WGS) of the Kp-48 ST307 strain, identified from urine-culture in a tertiary care hospital in Palermo, Italy, in May 2014 (5).
WGS was performed on the 454-GS Junior platform according to the standard sequencing procedure (Roche Diagnostics). Genomic coverage was 70×. Reads were aligned and assembled using gsAssembler v2.8 (Roche Diagnostics).
The complete sequence of Kp-48 revealed that the assembled genome size was 5,687,551 bp in length. Sequencing identified 4,395 annotated genes, 76 tRNAs, 25 rRNAs, and 719 open reading frames encoding uncharacterized proteins. Three extrachromosomal elements were found: the two IncFIIK plasmids pKpQIL (116,499 bp) and pKN3 (222,588 bp), and a novel phage (51,998 bp). The blaKPC-3 gene was localized on the pKpQIL plasmid, 99% identical to that described in ST258 isolates (4). The ST307 strain carries a novel variant of the pKN3 plasmid with the blaCTX-M-15, blaOXA-1, aac(6′)-Ib-cr, and qnrB1 resistance genes and two novel virulence clusters, encoding the enzymes for glycogen synthesis and a new urea transport system. An HK97-like phage was also present in the ST307 chromosome. The wzi-173 capsular antigen allele for this strain was exacerbated from the genome sequence, assigning ST307 to the previously described KN2 capsular type (6). Interestingly, the ST307 genome also carried a second capsular cluster, whose best match was found with the Enterobacter aerogenes genomes (GenBank accession numbers CP011574, FO203355, and CP002824). Putative VirB type IV secretion were identified in regions of major discordance with the ST258 genome (7).
The ST307 clone, carrying a novel assortment of resistance genes and new plasmid located virulence traits, may compete and prevail with the worldwide successful ST258 clone, further spreading the life-threatening KPC-producing K. pneumoniae isolates.
Nucleotide sequence accession number.
The whole-genome shotgun project of the KP-48 strain has been deposited at DDBJ/EMBL/GenBank under the accession no. LKAB00000000.
ACKNOWLEDGMENT
M.I. discloses that he has a permanent position as Sequencing Key Account Manager at Roche Tissue Diagnostic & Sequencing, Roche Diagnostic SPA, Monza, Italy. However, in this study he contributed to the assembly and annotation of the genome sequence using his precedent knowledge and experience in bioinformatics. There was no interference, personal interest, or any kind of economic relationship that might have detracted this author's objectivity with respect to the results presented in this study.
Footnotes
Citation Villa L, Feudi C, Fortini D, Iacono M, Bonura C, Endimiani A, Mammina C, Carattoli A. 2016. Complete genome sequence of KPC-3- and CTX-M-15-producing Klebsiella pneumoniae sequence type 307. Genome Announc 4(2):e00213-16. doi:10.1128/genomeA.00213-16.
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