Limbal stem cell transplantation: current perspectives

Abstract

Regeneration of the corneal surface after an epithelial insult involves division, migration, and maturation of a specialized group of stem cells located in the limbus. Several insults, both intrinsic and extrinsic, can precipitate destruction of the delicate microenvironment of these cells, resulting in limbal stem cell deficiency (LSCD). In such cases, reepithelialization fails and conjunctival epithelium extends across the limbus, leading to vascularization, persistent epithelial defects, and chronic inflammation. In partial LSCD, conjunctival epitheliectomy, coupled with amniotic membrane transplantation, could be sufficient to restore a healthy surface. In more severe cases and in total LSCD, stem cell transplantation is currently the best curative option. Before any attempts are considered to perform a limbal stem cell transplantation procedure, the ocular surface must be optimized by controlling causative factors and comorbid conditions. These factors include adequate eyelid function or exposure, control of the ocular surface inflammatory status, and a well-lubricated ocular surface. In cases of unilateral LSCD, stem cells can be obtained from the contralateral eye. Newer techniques aim at expanding cells in vitro or in vivo in order to decrease the need for large limbal resection that may jeopardize the “healthy” eye. Patients with bilateral disease can be treated using allogeneic tissue in combination with systemic immunosuppressive therapy. Another emerging option for this subset of patients is the use of noncorneal cells such as mucosal grafts. Finally, the use of keratoprosthesis is reserved for patients who are not candidates for any of the aforementioned options, wherein the choice of the type of keratoprosthesis depends on the severity of the disease. In summary, limbal stem cell transplantation improves both vision and quality-of-life in patients with ocular surface disorders associated with LSCD, and overall, the use of autologous tissue offers the best results. Future studies aim at improving cellular expansion and finding different sources of stem cells.

Introduction

The human ocular surface serves the unique function of forming a resilient barrier to pathogens and environmental factors, providing metabolic requirements to the underlying stroma, and maintaining a smooth transparent optical surface. It is composed of two main tissues: the cornea and the conjunctiva. A transition zone, the limbus, separates the two tissues. The limbus is composed of radial fibrovascular ridges – the palisades of Vogt – that form a niche for corneal epithelial stem cells (Figure 1). Regeneration of the corneal surface after an epithelial insult involves division, migration, and maturation of these cells.^1,2 Dua and Forrester³ described cell movement in reepithelialization as circumferential “tongue-shaped projections” that meet along the limbus first and then migrate centripetally to close any central defect. Lineage tracing can allow tracking of a stem cell and its progeny through the processes of cell division, differentiation, and distribution across the corneal surface.^4,5 Finding a definitive limbal epithelial stem cell marker, however, is a difficult task due to ambiguity in differentiating a stem cell from a progenitor and even from transit-amplifying cell.⁴ Promising markers for human limbal epithelial stem cells include the ATP-binding cassette (ABC) family members ABCB5⁶ and ABCG2,⁷ cytoskeletal intermediate filament proteins K14, K15, K19, and K3/K12.^8,9 Other markers identified include mediators of WNT and K14 pathways.^10,11

Figure 1.

Slit-lamp photograph of the palisades of Vogt at the limbus of an eye with a healthy ocular surface.

Note: Keratoplasty sutures can be seen inferiorly.

Limbal stem cell deficiency

Several insults, both intrinsic and extrinsic, can precipitate destruction of the delicate microenvironment of the stem cell niche. In the absence of structural support, the limbal stem cell population dies and the cornea loses its ability to regenerate itself; thus, scarring and loss of transparency occur. Causes of stem cell deficiency are summarized in Table 1, and they are divided into two groups depending on the severity of the ocular surface dryness that ensues. These are general categories as many times the degree of cicatrization due to each etiology runs a spectrum and it varies with the severity and extent of the insult. Upon sectoral destruction of the limbus, stem cells from adjacent limbal areas attempt to reepithelialize it. With more extensive severe insults, however, reepithelialization fails and conjunctival epithelium extends across the limbus, leading to vascularization, persistent epithelial defects, chronic inflammation (Figure 2).¹² Pathology and cytology show a corneal surface covered by conjunctival epithelium containing goblet cells.^13,14

Table 1.

Ocular conditions leading to limbal stem cell deficiency

Traumatic, iatrogenic, and malignant causes: more favorable	Inflammatory, hereditary, and neuropathic causes: unfavorable
• Chemical or thermal burn	• Stevens–Johnson syndrome
• Multiple surgeries	• Mucous membrane pemphigoid
• Radiation	• Chronic limbitis
• Antimetabolites	• Chronic bullous keratopathy
• Contact lens wear	• Neurotrophic keratopathy: trigeminal neuralgia, diabetes, herpes simplex, and zoster
• Infections	• Aniridia
• Neoplasia	• Epidermal dysplasia

Figure 2.

Limbal stem cell deficiency.

Note: Neovascularization, conjunctivalization, epithelial defects, and scarring are seen.

Clinically, patients with limbal stem cell deficiency (LSCD) present with pain, decreased vision, and photophobia. On examination, there is loss of the palisades of Vogt, a “whorled-like” corneal epithelium or frank conjunctivalization, scarring, and neovascularization in advanced cases. Poor adhesion of the epithelium causes recurrent erosions and persistent epithelial defects that can get secondarily infected.^15,16 Currently, there is no good diagnostic modality, and the diagnosis remains a clinical one. Corneal impression cytology may reveal goblet cells and confocal microscopy can confirm loss of the palisades of Vogt.^17,18

Treatment of LSCD

Management of patients with LSCD depends on the extent of involvement of the limbus (sectoral vs total) and on the unilaterality or bilaterality of the disease (Figure 3). For partial LSCD, mechanical debridement of the conjunctival epithelium from the surface of the cornea can be enough to restore a stable ocular surface as stem cells from the healthy limbal sectors divide and migrate to cover the defect. Scraping of the conjunctival epithelium can be coupled with amniotic membrane transplantation, which may allow for faster healing of the ocular surface.^17,19

Figure 3.

Pre- and postautologous stem cell transplantation photographs.

Notes: (A) Patient with limbal stem cell deficiency after chemical burn injury, with neovascularization and scarring (left) and donor eye with healthy stem cell niche (right). (B) Slit-lamp photograph after autologous stem cell transplant in affected (left) and donor eye (right) with evident wide excision. (C) Slit-lamp photograph after penetrating keratoplasty one year after initial stem cell transplant.

Management of patients with total LSCD has always been challenging because corneal clarity cannot be restored merely by a traditional corneal graft. Penetrating keratoplasty (PKP) is contraindicated in the setting of LSCD. For many years, treatment involved autologous (in cases of unilateral LSCD) or allogeneic keratolimbal grafts.^20–26 Recent advances in understanding limbal physiology and manipulating limbal stem cells ex vivo allow for the possibility of restoration of a healthy ocular surface with tissue-sparing surgery. This decreases the need for large limbal resection that may jeopardize the homeostasis of the “healthy” eye. These new tissue-sparing techniques also decrease the need for allogeneic tissue in some cases, thus eliminating the need for chronic immunosuppression.^22,27–31 Allogenic transplantation techniques have also seen improvement in outcomes with the advent of better immunosuppressive approaches and a better understanding of immunosuppression.

Optimization of the ocular surface

Prior to any attempt at limbal stem cell transplantation, the ocular surface must be optimized. Conservative first-line measures are based on two general principles: controlling causative factors, and controlling comorbid conditions. Causative factor control includes institution of immunosuppression for autoimmune diseases and/or chronic ocular surface inflammation, eradication of infection with appropriate antibiotic regimen, control of inflammation with corticosteroids, removal of any ocular surface tumor, and cessation of iatrogenic insults. Comorbid conditions such as aqueous tear deficiency, cicatricial changes of the eyelid and conjunctiva, trichiasis, and lagophthalmos should be managed preoperatively. The goal is to provide an optimal milieu for any existing stem cells to regenerate and the best-possible conditions for the transplanted stem cells to recover.¹⁷ Measures taken to improve lubrication include punctal occlusion, autologous serum tears, scleral lenses, and salivary gland implants. Lysis of symblephara and fornix reconstruction with mucous membrane grafting should be performed to reduce mechanical irritation caused by the eyelid. Repair of eyelid malpositions is necessary to eliminate chronic irritation due to trichiasis and to allow for better closure and maintenance of a stable tear film. In cases of persistent epithelial defects, Botox^®-induced ptosis or temporary tarsorrhaphy may be necessary.

Ocular surface stem cell transplantation techniques

Once an accurate diagnosis of LSCD is made and the ocular surface has been stabilized, limbal stem cell transplantation becomes the ultimate solution to restore the corneal epithelium. Various approaches are possible. Selection of the technique and its success prospects vary depending on the cause of LSCD, unilaterality or bilaterality of the deficiency, extent of LSCD (total vs partial), and the involvement of surrounding structures, namely, the conjunctiva and the eyelid. Great consideration is also given to patient-related factors such as burden of disease and expectations. The Cornea Society has proposed a classification for the various techniques of ocular surface stem cell transplantation, which is based on the following parameters: anatomic source of the transplanted tissue (conjunctival, keratolimbal, or mucosal); autologous or allogeneic (cadaveric or living-related) source; and cell culture techniques (Table 2).³²

Table 2.

Procedure nomenclature

Source	Tissue
	Conjunctival	Limbal	Keratolimbal
Autograft	CAU	CLAU	KLAU
Allograft – cadaveric	c-CAL	c-CLAL	KLAL
Allograft – living-related	lr-CAL	lr-CLAL	–
Allograft – living nonrelated	lnr-CAL	lnr-CLAL	–

Note: – Represents data not defined.

Abbreviations: c-CAL, cadaveric conjunctival allograft; CAU, conjunctival autograft; c-CLAL, cadaveric conjunctival limbal allograft; CLAU, conjunctival limbal autograft; lr-CAL, living-related conjunctival allograft; lr-CLAL, living-related conjunctival limbal allograft; lnr-CAL, living-nonrelated conjunctival allograft; lnr-CLAL, living-nonrelated conjunctival limbal allograft; KLAU, keratolimbal autograft; KLAL, keratolimbal allograft.

Traditional conjunctival autografts and conjunctival limbal autografts

The conjunctival limbal autograft (CLAU) procedure was one of the first curative techniques to be described for LSCD. It was first described by Jose Barraquer in the World Cornea Congress in 1964²² and revisited by Richard Thoft³³ in 1977 for unilateral ocular surface injuries. Better understanding of the physiology of the limbus over the next decade^27–30 allowed Kenyon and Tseng²⁰ to further develop this procedure in 1989. In this technique, which remains the treatment of choice for unilateral injuries, two large free grafts, each spanning from 5 mm to 7 mm of limbal arc length, that is 240°, are harvested from the normal eye and transplanted to the diseased eye (Figure 3).²⁰

CLAU is limited by the degree of LSCD in the affected eye and the risk of destabilizing the ocular surface in the good eye. It is thought that harvesting about 40% of stem cells would not destabilize the donor eye. With respect to outcomes, a review of the literature revealed that vision was improved in 90% of patients with unilateral total LSCD who underwent CLAU (n=39) and the ocular surface was restored in 94% of them when large grafts (>120°) were used. Visual improvement dropped down to 60% of cases (n=22) when smaller grafts were attempted to avoid jeopardizing the donor eye.¹⁷

Living-related conjunctival–limbal allograft

Kwitko et al²⁵ were the first to use conjunctival tissue from a living relative (parent or sibling) to manage LSCD in the procedure that is now known as living-related conjunctival allograft (Ir-CAL), which was then modified to include limbus along with conjunctiva (Ir-CLAL). Both Ir-CAL and Ir-CLAL are used to manage bilateral LSCD. Systemic immunosuppression is required to avoid rejection of the allograft. More recent advances include the use of trephines to harvest the conjunctival limbal grafts and fibrin glue to secure the grafts.^34,35

Cadaveric keratolimbal allografts

The keratolimbal allograft (KLAL) procedure uses cadaveric limbal tissue as the source of limbal stem cells, which thus allows for a larger stem cell supply. In the current version of this technique, two donor corneoscleral rims are used to restore a complete 360° limbus to the diseased eye.^36–38 This technique is reserved for patients with bilateral LSCD, for patients with no available or willing living relative for Ir-CLAL, and for patients with unilateral disease who are hesitant to have their only healthy eye as the source of limbal stem cells. Systemic immunosuppression is required for long-term graft survival, and despite immunosupression, outcomes are not optimal.^22,39,40 Adverse effects related to long-term immunosuppression after KLAL are frequent and include anemia, hyperglycemia, elevated creatinine, and elevated levels of liver function markers.^41,42

Autologous ex vivo cultivated limbal epithelial transplantation

Though the concept of cultured epithelial stem cell-based therapy was developed in the 1970s,⁴³ this technique was not applied for the treatment of ocular surface disease until 1997 by Pellegrini et al.⁴⁴ In this technique, epithelial stem cells are harvested by a small limbal biopsy from the donor contralateral eye and cultured ex vivo. Amniotic membrane or a fibrin-based substrate can be used as the carrier for the ex vivo culture of limbal stem cells that are autologous. Even though allogeneic (from living-related or deceased donors) cells have been used, their success rate is not as good as autologous cells.^45–47

Autologous ex vivo cultivated limbal epithelial transplantation (CLET) has been used successfully to treat unilateral, partial, or total LSCD. It is a technique that promises faster epithelialization and less inflammation, and it has the advantage of using significantly less amount of tissue from the donor eye than traditional CLAL. In this technique, a small 2×2 mm limbal biopsy is retrieved from the patient’s fellow healthy eye and the harvested cells are sent immediately for culture. Such small explants minimize any potential risk of LSCD to the healthy eye. Several culture techniques have been developed and can be divided into two general categories: explant or suspension methods. In the explant technique, a deepithelialized amniotic membrane is used as the scaffold for stem cell expansion and after 2–3 weeks, the composite graft is transplanted onto the diseased eye.^45,46 In the suspension method, the harvested limbal stem cells are first enzymatically treated and then seeded on a fibrin substrate carrier, amniotic membrane, or a layer of 3T3 fibroblasts that cover a plastic culture dish. Again, once the epithelial sheets become confluent, they are transferred onto the diseased eye.^44,48 Furthermore, any remaining expanded cells can be cryopreserved for potential future use. When allogeneic tissue is expanded, systemic immunosuppression is recommended for the recipient.

With respect to CLET outcomes, reported long-term results (mean follow-up: 1.5–8 years) vary, with success rates ranging from 47% to 100% for restoration of a stable ocular surface.^47–59 This variation may be due to poor optimization of the ocular surface prior to transplantation or due to the nature of the underlying disease process that led to LSCD. For instance, autoimmune diseases tend to recur with bouts of relentless inflammation that produce a dry ocular surface and jeopardize the graft. However, looking at a patient subset with LSCD and a wet ocular surface (eg, due to chemical or thermal burns, ocular surface malignancy, or surgical trauma), 66% (n=313) had improvement in visual acuity, and 79% (n=541) had a successful autologous graft.^47–59 Limitations of CLET include the high cost and the need for a good manufacturing practice and facility to properly process and expand the harvested limbal stem cells.

Simple limbal epithelial transplantation

In 2011, Sangwan et al⁶⁰ introduced simple limbal epithelial transplantation (SLET) as an alternative to CLET, a novel approach that achieves in vivo expansion of harvested limbal stem cells. In this technique, a small (2×2 mm) donor limbal graft from the unaffected eye is harvested and divided into smaller pieces, which are then expanded in vivo in the stem cell-deficient eye with the use of a fresh amniotic membrane and fibrin glue. The technique has been used to treat unilateral LSCD and has even been successfully attempted in bilateral partial LSCD. Modifications to this technique have been described, wherein two amniotic membrane layers are used to sandwich and protect the harvested limbal stem cells that are spirally distributed on the affected eye.⁶¹

Outcomes of this procedure are promising, with success rates similar to the rates of the above techniques and the added advantage of low cost and small harvest site (Figure 4). A multicenter study looked at 68 eyes of 68 patients who underwent SLET for unilateral LSCD. Clinical success, defined as a completely epithelialized, avascular, stable corneal surface, was achieved in 57 (84%) cases. With a median follow-up of 12 months, survival probability exceeded 80%.⁶²

Figure 4.

Simple limbal epithelial transplantation.

Notes: Patient with chemical burn with scarring conjunctivalization and neovascularization (left panel) and 6 months after simple limbal epithelial transplantation (right panel).

Cultivated oral mucosal epithelial transplantation

In the cultivated oral mucosal epithelial transplantation (COMET) technique, reconstruction of the ocular surface relies on the autologous epithelium of oral mucosal, rather than ocular, origin. This bypasses the need for an allograft in patients with bilateral disease and is, thus, a promising replacement for KLAL or allogeneic CLET, both of which require long-term systemic immunosuppression.

A healthy oral mucosa is examined by a dentist or maxillofacial surgeon and a 2–3 mm² biopsy is cut into small explants and cultured on a denuded amniotic membrane for ~2–3 weeks so that a confluent epithelial sheet is produced.^63,64 At the time of the procedure, corneal pannus is removed, and mitomycin C (0.04%) is applied for 5 minutes and washed thoroughly before the amniotic membrane with the explants is secured with a 10-0 nylon suture at the limbus. A bandage contact lens is applied afterward.^63–66

Sotozono et al⁶⁷ reported good long-term visual outcomes (mean follow-up: 2 years) in about 50% of 15 patients who underwent COMET for bilateral LSCD. Satake et al⁶⁸ performed COMET on 40 eyes and achieved a 57.5% overall success rate, at a mean follow-up interval of 25.5 months. Failure was due to persistent epithelial defects in nine eyes and gradual fibrovascular tissue invasion of the corneal surface in eyes with mucous membrane pemphigoid.

Emerging techniques are investigating the potential of reprogramming cells from various sources obtained through other minimally invasive techniques.^69,70 Induced pluripotent stem cells obtained in this manner are then differentiated into limbal stem cells.⁷¹

Limbal stem cell transplantation and secondary keratoplasty

The primary goal of limbal stem cell transplantation is to restore a stable ocular surface. Vision may improve, but many times, a secondary keratoplasty is needed to restore corneal clarity and its success is dependent on the presence of limbal stem cells. Solomon et al⁷² compared 23 eyes that underwent simultaneous KLAL and PKP with 16 eyes that underwent KLAL alone. Ambulatory vision was better at 2 years for eyes that underwent KLAL alone (86.1%±9.1%) than KLAL with PKP (46.9%±10.6%). Survival of PKP may be better if performed after the limbus is restored by KLAL rather than at the time of primary KLAL surgery. Overall survival of KLAL was 76.9%±6.7% at 1 year, 47.4%±11.7% at 3 years, and only 23.7%±17.7% at 5 years. Central corneal graft survival was 47.8%±10.4% at 1 year and 13.7%±8.4% at 3 years; it was significantly worse (P-value: 0.028) in eyes with Stevens–Johnson syndrome (SJS) (20.0%±17.9%) compared with eyes affected by other causes (55.6%±11.7%).

Basu et al⁷³ followed 47 patients who underwent PKP either at the time of CLET (single-stage procedure) or >6 weeks later (two-stage procedure) for an average of 4.2±1.9 years. Overall allograft survival at 1 year was 66%±7%, with significantly better (P-value: 0.0003) survival for eyes that underwent a two-stage procedure (80%±6%; median survival: 4 years) compared to a single-stage one (25%±13%; median survival: 6 months). There was no difference in outcomes for eyes that underwent PKP between 6 weeks and 6 months after CLET and those that had it >6 months after CLET. Over the whole study, allografts failed in 55.3% of cases due to rejection (57%), central graft infiltrates (26.9%), and LSCD recurrence (15.4%).

A multicenter study followed a subset of nine patients who underwent SLET with keratoplasty. A completely epithelialized, avascular, stable corneal surface was achieved in five cases (55.6%); however, follow-up was limited as the procedure is rather new (Figure 5).⁶²

Figure 5.

Combined penetrating keratoplasty and simple limbal epithelial transplantation.

Notes: Patient with history of Acanthamoeba infection, with scarring and limbal stem cell deficiency (left panel). Patient underwent simple limbal epithelial transplantation and penetrating keratoplasty and 6 months postoperative status of the eye is shown (right panel).

Satake et al⁶⁸ performed COMET with PKP on seven eyes, with a mean period between the two of 12.6 months and follow-up interval of 22.6 months after keratoplasty.⁶⁸ The epithelium was maintained in six eyes, two of which showed conjunctival invasion after 18 months. Corneal clarity was reportedly maintained in four eyes (57.1%).

Keratoprosthesis in LSCD

Visual rehabilitation in patients with LSCD is possible with the use of a keratoprosthesis. The Boston keratoprosthesis type 1 (Boston KPro type 1) is a good surgical option for patients with bilateral LSCD who are not candidates for immunosuppression or who have failed a limbal stem cell allograft. The Boston KPro type 1 offers good visual rehabilitation and good retention rate in patients with LSCD who have a wet ocular surface and good eyelid function. Sejpal et al⁷⁴ reported their experience in the management of patients with LSCD with the Boston KPro type 1. They included all patients with LSCD who received a KPro type 1, including patients with SJS with a relatively wet ocular surface. The authors concluded that for patients with bilateral LSCD with nonautoimmune etiology, the Boston KPro type 1 was a good alternative for rehabilitating vision in one eye.⁷⁴ The Boston KPro type 1 has also been used in patients who have failed a KLAL. Hou et al⁷⁵ reported a group of seven patients who failed KLAL and after a year of follow-up, all patients except one retained the Boston Kpro type 1. One patient failed due to sterile corneal necrosis and required a repeat keratoprosthesis surgery.⁷⁵ The most common complications of the Boston Kpro type 1 in the setting of LSCD are recurrent epithelial defects, retroprosthetic membrane formation, sterile melts, and secondary glaucoma. Sight-threatening complications such as endophthalmitis and retinal detachment have been described as well.

Patients with bilateral LSCD and a dry ocular surface, such as patients with end-stage SJS and burnt-out mucous membrane pemphigoid, are not candidates for limbal transplantation. For this group of patients, the best currently available options to rehabilitate vision are the Boston KPro type 2, the modified osteo-odonto keratoprosthesis (MOOKP), the Temprano keratoprosthesis, and the recently described LVP keratoprosthesis.⁷⁶ All of these keratoprosthesis models use the eyelids or buccal mucosa as a barrier of protection for the keratoprosthesis to improve retention rates. Long-term retention rates are poor for the Boston KPro type 2.⁷⁷ They are not available yet for the LVP keratoprosthesis.

The MOOKP is a staged procedure described first by Strampelli⁷⁸ and later modified by Falcinelli et al.⁷⁹ This keratoprosthesis utilizes a bone lamina from the patient’s tooth as a carrier for the optical cylinder and oral buccal mucosa to protect the lamina–optical cylinder complex. The retention rates have been described at about 96% after 1 year and 66% after 10 years.⁷⁹ The Temprano keratoprosthesis utilizes a similar concept as the MOOKP, but instead of tooth bone, this prosthesis utilizes tibial bone as the carrier for the optical cylinder. It also uses buccal mucosa to protect the bone/optical cylinder complex. The Temprano keratoprosthesis offers good long-term retention rates, similar to the MOOKP.⁸⁰ Even though both the MOOKP and the Temprano keratoprosthesis offer good long-term anatomical retention results, complications such as oroantral fistula, trophic mucosal alterations, lamina exposure, mucous membrane overgrowth, hypotony, expulsion of optic cylinder, endophthalmitis glaucoma, sterile vitritis, and retinal detachment have been well described.

Conclusion

In the past 3 decades, significant progress has been made in understanding the physiology of the limbal epithelial stem cells and their key role in maintaining corneal transparency. Currently, the prognosis of patients with unilateral LSCD and a wet ocular surface is very good. Current techniques allow for harvesting of cells from the “healthy donor eye” to restore the ocular surface of the “diseased eye” with acceptable risks. Even though significant progress has been made to improve the prognosis of patients with bilateral LSCD, with the currently available surgical techniques, this group of patients still needs to be under a regimen of systemic immunosuppression. Though immunosuppressive regimens can prevent rejection and help patients maintain corneal transparency, unfortunately, some patients are unable to tolerate these regimens over the long-term as they develop side effects that require cessation of the medications. For this reason, tissue engineering or newer tissue culturing techniques are bound to play a significant role in the future because the goal is to develop nonimmunogenic tissues that decrease or eliminate the need for systemic immunosuppression. One promising alternative is the use of induced pluripotent stem cells (iPSCs). Recently, Hayashi et al⁸¹ successfully generated corneal epithelial cells differentiated from human adult dermal fibroblast–derived and limbal epithelial cell–derived iPSCs. Gomes et al⁸² used a tissue-engineered cell sheet composed of human dental pulp stem cells for ocular surface reconstruction in a rabbit model of total LSCD. Successful engineering of such types of cells will allow patients with LSCD to use their own tissue to restore the limbal deficiency and avoid the need for immunosuppression. It is important to note that even with this potential treatment, presurgical planning will still play a catalytic role for the success of such procedures. A wet ocular surface, adequate eyelid function, and control of the ocular surface inflammatory status needs to be restored before attempting any type of surgical rehabilitation for patients with LSCD.