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. 1992 Jan 1;89(1):449–451. doi: 10.1073/pnas.89.1.449

An rbcL sequence from a Miocene Taxodium (bald cypress).

P S Soltis 1, D E Soltis 1, C J Smiley 1
PMCID: PMC48255  PMID: 1729716

Abstract

During the past decade, ancient DNAs from both animals and plants have been successfully extracted and analyzed. Recently, the age of DNA that can be recovered and sequenced was increased manyfold by the amplification and sequencing of a DNA fragment from a Magnolia fossil obtained from the Miocene Clarkia deposit (17-20 million yr old). However, the validity of this report has been questioned based on models predicting that DNA should be completely degraded after 4 million yr. We report here the successful amplification, sequencing, and analysis of a 1320-base-pair portion of the chloroplast gene rbcL from a Miocene Taxodium specimen, also from the Clarkia site. These data not only validate the earlier report of sequence data for a Magnolia species from the same site but also suggest that it may be possible to isolate and sequence DNAs routinely from the Clarkia deposit. The ability to recover and sequence DNAs of such age offers enormous research possibilities in the areas of molecular evolution, biogeography, and systematics.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol. 1981;17(6):368–376. doi: 10.1007/BF01734359. [DOI] [PubMed] [Google Scholar]
  2. Fitch W. M., Margoliash E. Construction of phylogenetic trees. Science. 1967 Jan 20;155(3760):279–284. doi: 10.1126/science.155.3760.279. [DOI] [PubMed] [Google Scholar]
  3. Golenberg E. M. Amplification and analysis of Miocene plant fossil DNA. Philos Trans R Soc Lond B Biol Sci. 1991 Sep 30;333(1268):419–427. doi: 10.1098/rstb.1991.0092. [DOI] [PubMed] [Google Scholar]
  4. Golenberg E. M., Giannasi D. E., Clegg M. T., Smiley C. J., Durbin M., Henderson D., Zurawski G. Chloroplast DNA sequence from a miocene Magnolia species. Nature. 1990 Apr 12;344(6267):656–658. doi: 10.1038/344656a0. [DOI] [PubMed] [Google Scholar]
  5. Higuchi R., Bowman B., Freiberger M., Ryder O. A., Wilson A. C. DNA sequences from the quagga, an extinct member of the horse family. Nature. 1984 Nov 15;312(5991):282–284. doi: 10.1038/312282a0. [DOI] [PubMed] [Google Scholar]
  6. Hipkins V. D., Tsai C. H., Strauss S. H. Sequence of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from a gymnosperm, Douglas fir. Plant Mol Biol. 1990 Sep;15(3):505–507. doi: 10.1007/BF00019168. [DOI] [PubMed] [Google Scholar]
  7. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980 Dec;16(2):111–120. doi: 10.1007/BF01731581. [DOI] [PubMed] [Google Scholar]
  8. Kreitman M., Landweber L. F. A strategy for producing single-stranded DNA in the polymerase chain reaction. A direct method for genomic sequencing. Gene Anal Tech. 1989 Jul-Aug;6(4):84–88. doi: 10.1016/0735-0651(89)90021-6. [DOI] [PubMed] [Google Scholar]
  9. Lindahl T., Andersson A. Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleic acid. Biochemistry. 1972 Sep 12;11(19):3618–3623. doi: 10.1021/bi00769a019. [DOI] [PubMed] [Google Scholar]
  10. Lindahl T., Nyberg B. Rate of depurination of native deoxyribonucleic acid. Biochemistry. 1972 Sep 12;11(19):3610–3618. doi: 10.1021/bi00769a018. [DOI] [PubMed] [Google Scholar]
  11. Päbo S., Gifford J. A., Wilson A. C. Mitochondrial DNA sequences from a 7000-year old brain. Nucleic Acids Res. 1988 Oct 25;16(20):9775–9787. doi: 10.1093/nar/16.20.9775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Päbo S. Molecular cloning of Ancient Egyptian mummy DNA. Nature. 1985 Apr 18;314(6012):644–645. doi: 10.1038/314644a0. [DOI] [PubMed] [Google Scholar]
  13. Päbo S., Wilson A. C. Miocene DNA sequences - a dream come true? Curr Biol. 1991 Feb;1(1):45–46. doi: 10.1016/0960-9822(91)90125-g. [DOI] [PubMed] [Google Scholar]
  14. Zurawski G., Clegg M. T., Brown A. H. The Nature of Nucleotide Sequence Divergence between Barley and Maize Chloroplast DNA. Genetics. 1984 Apr;106(4):735–749. doi: 10.1093/genetics/106.4.735. [DOI] [PMC free article] [PubMed] [Google Scholar]

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