Extended Data Table 1. List of the 48 proteins exclusive to MDA-MB-231 exosomes.
Listing of the 48 proteins exclusively detected in exosomes from MDA-MB-231 cells determined by ultra performance liquid chromatography – mass spectrometry (UPLC-MS) and comparative analyses of exosomes derived from NIH/3T3, MCF 10A, HDF, E10 and MDA-MB-231 cells. The proteins are grouped based on cellular location.
| Protein Name | Gene ID | Cellular Location |
|---|---|---|
| ATP-binding cassette sub-family A member 6 | ABCA6 | Transmembrane |
| Tetraspanin-4 | TSPAN4 | Transmembrane |
| SLIT and NTRK-like protein 4 | SLITRK4 | Transmembrane |
| Putative protocadherin beta-18 | PCDHB18 | Transmembrane |
| Myeloid cell surface antigen CD33 | CD33 | Transmembrane |
| Glypican-1 | GPC1 | Membrane anchored |
| Protein Name | Gene ID | Cellular Location |
|---|---|---|
| Histone H2A type 2-A | HIST1H2AA | Nucleus |
| Histone H2A type 1-A | HIST1H1AA | Nucleus |
| Histone H3.3 | H3F3A | Nucleus |
| Histone H3.1 | HIST1H3A | Nucleus |
| Zinc finger protein 37 homolog | ZFP37 | Nucleus |
| Hypermethylated in cancer 2 protein | HIC2 | Nucleus |
| Zinc finger protein 12 | ZSCAN12 | Nucleus |
| Protein Name | Gene | ID Cellular Location |
|---|---|---|
| Laminin subunit beta-1 | LAMB1 | Secreted |
| Tubulointerstitial nephritis antigen-like | TINAGL1 | Secreted |
| Peroxiredoxin-4 | PRDX4 | Secreted |
| Collagen alpha-2(IV) chain | COL4A2 | Secreted |
| Putative protein C3P1 | C3P1 | Secreted |
| Collagen alpha-1(II) chain | COL2A1 | Secreted |
| Hemicentin-1 | HMCN1 | Secreted |
| Protein Name | Gene ID | Cellular Location |
|---|---|---|
| Putative rhophilin-2-like protein | RHPN2P1 | Not specified |
| Ankyrin repeat domain-containing protein 62 | ANKRD62 | Not specified |
| Tripartite motif-containing protein 42 | TRIM42 | Not specified ! |
| Protein Name | Gene ID | Cellular Location |
|---|---|---|
| Junction plakoglobin | JUP | Cytoplasm |
| Tubulin beta-2B chain | TUBB2B | Cytoplasm |
| Endoribonuclease Dicer | DICER1 | Cytoplasm |
| E3 ubiquitin-protein ligase TRIM71 | TRIM71 | Cytoplasm |
| Katanin p60 ATPase-containing subunit A-like 2 | KATNAL2 | Cytoplasm |
| Protein S100-A6 | S100A6 | Cytoplasm |
| 5′-nucleotidase domain-containing protein 3 | NT5DC3 | Cytoplasm |
| Valine--tRNA ligase | VARS | Cytoplasm |
| Kazrin | KAZN | Cytoplasm |
| ELAV-like protein 4 | ELAVL4 | Cytoplasm |
| RING finger protein 166 | RNF166 | Cytoplasm |
| FERM and PDZ domain-containing protein 1 | FRMPD1 | Cytoplasm |
| 78 kDa glucose-regulated protein | HSPA5 | Cytoplasm |
| Trafficking protein particle complex subunit 6A | TRAPPC6A | Cytoplasm |
| Squalene monooxygenase | SQLE | Cytoplasm |
| Tumor susceptibility gene 101 protein | TSG101 | Cytoplasm |
| Vacuolar protein sorting 28 homolog | VPS28 | Cytoplasm |
| Prostaglandin F2 receptor negative regulator | PTGFRN | Cytoplasm |
| Isobutyryl-CoA dehydrogenase, mitochondrial | ACAD8 | Cytoplasm |
| 26S protease regulatory subunit 6B | PSMC4 | Cytoplasm |
| Elongation factor 1-gamma | EEF1G | Cytoplasm |
| Titin | TTN | Cytoplasm |
| Tyrosine-protein phosphatase type 13 | PTPN13 | Cytoplasm |
| Triosephosphate isomerase | TPI1 | Cytoplasm |
| Carboxypeptidase E | CPE | Cytoplasm |