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. 2015 Dec 24;7(4):4399–4413. doi: 10.18632/oncotarget.6749

Figure 2. Sildenafil and DOX co-treatment reduced FLIP expression in DU145 cells.

Figure 2

Representative images of the immunohistochemical staining for FLIP in DU145 cells following 48 hr treatment with DOX (0.5 μM) and/or Sild (10 μM). The paraformaldehyde fixed cells were incubated with rabbit FLIP-s and mouse tubulin antibodies and visualized by Alexa Fluor 488-conjugated anti-rabbit IgG (green fluorescence) and Alexa 555-conjugated anti-mouse IgG (red fluorescence), then examined by confocal microscopy. Abbreviations: doxorubicin – DOX; Sildenafil – Sild.