Table 1.
Summary of the effects of mutations in IE62 domains I–III.
| IE62 domain | Mutation | Effect on virus replication kinetics | Effect on IE62 localization | Effect on IE62 and IE63 expression |
|---|---|---|---|---|
| Domain I | -F50A | No effect | NA | NA |
| -aa57-60 mutant | No effect | NA | NA | |
| -Δ238–258 | No effect | NA | NA | |
| Domain II | -K548E | Lethal | NA | NA |
| -Δ495–528 | Lethal | NA | NA | |
| Domain III | -NLS mutant | Inhibition at days 1–2 post infection | IE62 predominant in cytoplasm | Low accumulation of IE62 and IE63 |
| -S686A/S722A | Inhibition at days 4–5 post infection | IE62 predominant in nucleus | Low accumulation of IE62 and IE63 |