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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1992 Jan 15;89(2):559–563. doi: 10.1073/pnas.89.2.559

Characterization of a prototype strain of hepatitis E virus.

S A Tsarev 1, S U Emerson 1, G R Reyes 1, T S Tsareva 1, L J Legters 1, I A Malik 1, M Iqbal 1, R H Purcell 1
PMCID: PMC48278  PMID: 1731327

Abstract

A strain of hepatitis E virus (SAR-55) implicated in an epidemic of enterically transmitted non-A, non-B hepatitis, now called hepatitis E, was characterized extensively. Six cynomolgus monkeys (Macaca fascicularis) were infected with a strain of hepatitis E virus from Pakistan. Reverse transcription-polymerase chain reaction was used to determine the pattern of virus shedding in feces, bile, and serum relative to hepatitis and induction of specific antibodies. Virtually the entire genome of SAR-55 (7195 nucleotides) was sequenced. Comparison of the sequence of SAR-55 with that of a Burmese strain revealed a high level of homology except for one region encoding 100 amino acids of a putative nonstructural polyprotein. Identification of this region as hypervariable was obtained by partial sequencing of a third isolate of hepatitis E virus from Kirgizia.

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Selected References

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  1. Andzhaparidze A. G., Balaian M. S., Savinov A. P., Braginskii D. M., Poleshchuk V. F. Vosproizvedenie na obez'ianakh gepatita ni A, ni B, peredaiushchegosia fekal'no-oral'nym putem. Vopr Virusol. 1986 Jan-Feb;31(1):73–81. [PubMed] [Google Scholar]
  2. Arankalle V. A., Ticehurst J., Sreenivasan M. A., Kapikian A. Z., Popper H., Pavri K. M., Purcell R. H. Aetiological association of a virus-like particle with enterically transmitted non-A, non-B hepatitis. Lancet. 1988 Mar 12;1(8585):550–554. doi: 10.1016/s0140-6736(88)91351-7. [DOI] [PubMed] [Google Scholar]
  3. Balayan M. S., Andjaparidze A. G., Savinskaya S. S., Ketiladze E. S., Braginsky D. M., Savinov A. P., Poleschuk V. F. Evidence for a virus in non-A, non-B hepatitis transmitted via the fecal-oral route. Intervirology. 1983;20(1):23–31. doi: 10.1159/000149370. [DOI] [PubMed] [Google Scholar]
  4. Bradley D. W., Balayan M. S. Virus of enterically transmitted non-A, non-B hepatitis. Lancet. 1988 Apr 9;1(8589):819–819. doi: 10.1016/s0140-6736(88)91674-1. [DOI] [PubMed] [Google Scholar]
  5. Bradley D. W. Enterically-transmitted non-A, non-B hepatitis. Br Med Bull. 1990 Apr;46(2):442–461. doi: 10.1093/oxfordjournals.bmb.a072409. [DOI] [PubMed] [Google Scholar]
  6. Bradley D. W., Krawczynski K., Cook E. H., Jr, McCaustland K. A., Humphrey C. D., Spelbring J. E., Myint H., Maynard J. E. Enterically transmitted non-A, non-B hepatitis: serial passage of disease in cynomolgus macaques and tamarins and recovery of disease-associated 27- to 34-nm viruslike particles. Proc Natl Acad Sci U S A. 1987 Sep;84(17):6277–6281. doi: 10.1073/pnas.84.17.6277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bradley D., Andjaparidze A., Cook E. H., Jr, McCaustland K., Balayan M., Stetler H., Velazquez O., Robertson B., Humphrey C., Kane M. Aetiological agent of enterically transmitted non-A, non-B hepatitis. J Gen Virol. 1988 Mar;69(Pt 3):731–738. doi: 10.1099/0022-1317-69-3-731. [DOI] [PubMed] [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gupta H., Tandon B. N., Sriramachari S., Joshi Y. K., Iyenger B. Animal transmission of enteric non-A, non-B hepatitis infection to Macaca mulatta by faeco-oral route. Indian J Med Res. 1990 Mar;91:87–90. [PubMed] [Google Scholar]
  10. Iqbal M., Ahmed A., Qamar A., Dixon K., Duncan J. F., Islam N. U., Rauf A., Bryan J. P., Malik I. A., Legters L. J. An outbreak of enterically transmitted non-A, non-B hepatitis in Pakistan. Am J Trop Med Hyg. 1989 Apr;40(4):438–443. doi: 10.4269/ajtmh.1989.40.438. [DOI] [PubMed] [Google Scholar]
  11. Krawczynski K., Bradley D. W. Enterically transmitted non-A, non-B hepatitis: identification of virus-associated antigen in experimentally infected cynomolgus macaques. J Infect Dis. 1989 Jun;159(6):1042–1049. doi: 10.1093/infdis/159.6.1042. [DOI] [PubMed] [Google Scholar]
  12. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  13. Neill J. D. Nucleotide sequence of a region of the feline calicivirus genome which encodes picornavirus-like RNA-dependent RNA polymerase, cysteine protease and 2C polypeptides. Virus Res. 1990 Nov;17(3):145–160. doi: 10.1016/0168-1702(90)90061-f. [DOI] [PubMed] [Google Scholar]
  14. Panda S. K., Datta R., Kaur J., Zuckerman A. J., Nayak N. C. Enterically transmitted non-A, non-B hepatitis: recovery of virus-like particles from an epidemic in south Delhi and transmission studies in rhesus monkeys. Hepatology. 1989 Oct;10(4):466–472. doi: 10.1002/hep.1840100411. [DOI] [PubMed] [Google Scholar]
  15. Reyes G. R., Purdy M. A., Kim J. P., Luk K. C., Young L. M., Fry K. E., Bradley D. W. Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science. 1990 Mar 16;247(4948):1335–1339. doi: 10.1126/science.2107574. [DOI] [PubMed] [Google Scholar]
  16. Reyes G. R., Yarbough P. O., Tam A. W., Purdy M. A., Huang C. C., Kim J. S., Bradley D. W., Fry K. E. Hepatitis E virus (HEV): the novel agent responsible for enterically transmitted non-A, non-B hepatitis. Gastroenterol Jpn. 1991 Jul;26 (Suppl 3):142–147. doi: 10.1007/BF02779285. [DOI] [PubMed] [Google Scholar]
  17. Skidmore S. J., Yarbough P. O., Gabor K. A., Tam A. W., Reyes G. R., Flower A. J. Imported hepatitis E in UK. Lancet. 1991 Jun 22;337(8756):1541–1541. doi: 10.1016/0140-6736(91)93227-z. [DOI] [PubMed] [Google Scholar]
  18. Smith D. B., Johnson K. S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31–40. doi: 10.1016/0378-1119(88)90005-4. [DOI] [PubMed] [Google Scholar]
  19. Soe S., Uchida T., Suzuki K., Komatsu K., Azumi J., Okuda Y., Iida F., Shikata T., Rikihisa T., Mizuno K. Enterically transmitted non-A, non-B hepatitis in cynomolgus monkeys: morphology and probable mechanism of hepatocellular necrosis. Liver. 1989 Jun;9(3):135–145. doi: 10.1111/j.1600-0676.1989.tb00390.x. [DOI] [PubMed] [Google Scholar]
  20. Tam A. W., Smith M. M., Guerra M. E., Huang C. C., Bradley D. W., Fry K. E., Reyes G. R. Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology. 1991 Nov;185(1):120–131. doi: 10.1016/0042-6822(91)90760-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Uchida T., Suzuki K., Komatsu K., Iida F., Shikata T., Rikihisa T., Mizuno K., Soe S., Win K. M., Nakane K. Occurrence and character of a putative causative virus of enterically-transmitted non-A, non-B hepatitis in bile. Jpn J Exp Med. 1990 Feb;60(1):23–29. [PubMed] [Google Scholar]
  22. Uchida T., Win K. M., Suzuki K., Komatsu K., Iida F., Shikata T., Rikihisa T., Mizuno K., Soe S., Myint H. Serial transmission of a putative causative virus of enterically transmitted non-A, non-B hepatitis to Macaca fascicularis and Macaca mulatta. Jpn J Exp Med. 1990 Feb;60(1):13–21. [PubMed] [Google Scholar]
  23. Yarbough P. O., Tam A. W., Fry K. E., Krawczynski K., McCaustland K. A., Bradley D. W., Reyes G. R. Hepatitis E virus: identification of type-common epitopes. J Virol. 1991 Nov;65(11):5790–5797. doi: 10.1128/jvi.65.11.5790-5797.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

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