Abstract
Nanoparticles have been used for many functional materials in nano-sciences and photo-catalyzing surface chemistry. The titanium oxide nanoparticles will be useful for the treatment of tumor by laser and/or ultrasound as the sensitizers in nano-medicine. We have studied the combination therapy of photo- and sono-dynamic therapies in an animal tumor model. Oral-administration of two sensitizers titanium oxide, 0.2%-TiO2 nanoparticles for sono-dynamic and 1 mM 5-aminolevulinic acid for photodynamic therapies have resulted in the best combination therapeutic effects for the cancer treatment. Our light microscopic and Raman spectroscopic studies revealed that the titanium nanoparticles were distributed inside the blood vessel of the cancer tissue (1–3 μm sizes). Among these nanoparticles with a broad size distribution, only particular-sized particles could penetrate through the blood vessel of the cancer tissue, while other particles may only exhibit the side effects in the model mouse. Therefore, it may be necessary to separate the optimum size particles. For this purpose we have separated TiO2 nanoparticles by countercurrent chromatography with a flat coiled column (1.6 mm ID) immersed in an ultrasonic bath (42 KHz). Separation was performed with a two-phase solvent system composed of 1-butanol-acetic acid-water at a volume ratio of 4:1:5 at a flow rate of 0.1 ml/min. Countercurrent chromatographic separation yielded fractions containing particle aggregates at 31 and 4400 nm in diameter.
Keywords: Titanium Dioxide (TiO2) Nanoparticles, 5-aminolevulinic Acid (5-ALA), Countercurrent Chromatography (CCC), Raman Spectrum Microscope, Sono-dynamic Therapy (SDT), Photo-dynamic Therapy
1. Introduction
Cancer could be treated as the most complicated disease in the medical science and for this reason scientists of different parts of the world are still trying their level best to achieve a full control on this chaotic pathological condition. Cancer cell can camouflage due to its resembling features with the host cell and therefore tracking down its abnormal characteristics seems to be the most challenging parts to succeed in its treatment. Another miserable situation in cancer cells is their unique and unpredictable molecular modifications through point mutations, translocations, fusions, and other aberrations even for the similar category of cancer in different individuals [1–5]. At present, however on many cases of cancer treatment scientists have managed quite satisfactorily through designing appropriate targeted therapy alone or with other conventional cancer treatment modalities like surgery, radiation therapy, and/or chemotherapy, etc. [6–10]. But the treatment spectrum of combination therapy is quite large and less expensive. Hence, scientists put their effort on this field in a great extent now a days and trying to generate effective as well as safer combination therapy using different means of combinations among surgery, radiation therapy, and/or chemotherapy, etc. In many cases drug-cocktails or sequenced therapeutic combinations of natural, synthetic or semi-synthetic chemotherapeutic agents have shown a great promise for treating cancer more effectively by avoiding or overcoming a very usual phenomenon of single drug induced resistance capability of the cancer cells. [11–15] In our study we have used the combinations of two different physical inputs of sound and light for activating a combined anticancer sensitizer, namely TiO2+ 5-ALA, more effectively.
Over the last 30 years sonodynamic therapy (SDT) has been built up and N. Miyoshi also has a very good research track on this technology regarding its application on cancer model [16–18]. Sonodynamic activity actually creates the acoustic cavitation effect in presence of some particles to generate hydroxyl radicals or singlet oxygen species into the biological environment and also enhance the cytotoxic property of some compounds. Another advantageous point is that it can be applied for deeper portion of the tissue and thereby it might be very much helpful on striking the root of the localized cancer. Hence, without any surgical operation this therapy can produce a high impact on inhibiting the tumor growth. Moreover, the multiple or frequent application is quite harmless with this kind of therapy.
On the other hand, photodynamic therapy (PDT) has also been regarded as a very effective means of cancer treatment mainly for the superficial origins like skin, esophagus, oral cavity, etc. [19–22] PDT, using a photosensitizer and laser emission, has been applied quite successfully in Japan for the last 15 years against the early cancers of esophagus, lung, stomach and cervix of the uterus. For the head and neck cancer also it has shown a good promise [23–26]. PDT is also very much safe in respect of adverse effects to the other biological parts around the cancer region. But, penetration depth of the light is limited and that’s why in case of advanced stage of a cancerous proliferation PDT often produces less success.
To overcome the aforementioned limitations of these two treatment modalities, we have carried out for the first time a combination therapy of PDT and SDT for the treatment of cancer model tissue. For this purpose, we have chosen titanium oxide, TiO2nanoparticles and 5-ALA (5-aminolevulinic acid) as the higher affected precursor of sensitizers for cancers. Here the 5-ALA directed the movement of TiO2 to the target tumor tissue. Recently, nanoparticles have been being used for many functional materials in nano-sciences [27–31] and photo-catalyzing surfaces [32–46]. The TiO2 particles (crystal size 5–6 nm which were used for UV-cut raw materials in a make-up) were previously used as the sono-sensitizer for the production of ·OH radicals by the ultrasound irradiation due to the cavitation effect with the particles in the water [18, 47–49].
From different studies it is also quite evident that movement or penetration of the nanoparticles depends largely on their particle size and agglomeration tendency with the surrounding biological components when applied endogenously [50–51]. Both intra- and inter-particular adherence might hinder their free or desired movement into the blood and thereby to the tissue. From this point of view, we have tried to separate different particles of 5-ALA/TiO2 aggregates with the help of a revolutionary liquid-liquid partition chromatography system, namely countercurrent chromatography (CCC), which utilizes a liquid stationary phase to avoid the sample loss due to irreversible adsorption or denaturation caused by the solid support used in conventional chromatographic module like HPLC or TLC [52–54]. In this method solutes are partitioned between mobile or stationary phases according to their partition coefficients, whereas, the aggregated particles are usually accumulated in the interphase of the immiscible droplets and eluted earlier. In our present study, we have generated smaller droplets in the CCC column using ultrasonic irradiation to improve the partitioning of aggregates of 5-ALA/TiO2.
Through our present study we also put our effort to understand the distribution pattern of the TiO2 in the tumor tissue to get some impression regarding the necessity of appropriate and regulated size of nanoparticles to be used as more effective sensitizers for this kind of physical input oriented modality of the anticancer treatment.
2. Experimental
2.1. Chemical Materials
5-Aminolevulinic acid hydrochloride salt (5-ALA; purity: 99.9%) used in this study was presented from Cosmo Oil Co, Ltd. (Tokyo, Japan). The nanoparticle of titanium oxide (TiO2) used in this study were also given from Tayca Co. Ltd. (Osaka, Japan) as the 20% colloidal solution of titanium dioxide TiO2 phosphate salt crystal (TKS-203: Ti3(PO4)4; the particle size is 5–6 nm which were used for a make-up as the raw materials). 1-Butanol of HPLC grade was purchased from Fisher Scientific, Park Lawn, NJ, USA, and acetic acid of analytical grade from Mallinckrodt, MO, USA. H & E histological staining solution was purchased from Katayama Chemical Engineering, Osaka, Japan.
2.2. Ultrasound Probe and the Instruments System: [Figure 1]
Ultrasound Probe (P) was assembled with a Cooler (C), an Amplifier (A) and a Generator (G) as a systematic instrument. The ultrasound irradiation instrument system at 1 M-hertz (MHz) had been built up according to the advice of Dr. Shinichiro Umemura of the Central Institute of Hitachi Ltd. Co. (Tokyo, Japan). Especially, the irradiation was adjusted by a special small probe (P: the diameter was 25mm) of ultrasound for the tumor model tissue (the diameter was within 10 mm, the irradiation time was 10 min) of BALB-c-nu/nu strain nude mice (20 g) during more than 5 years of a collaboration research with the institute as shown in Figure 1.
2.3. Animal Experiments: [Figure 2]
A C3H/He-strain mouse (CLEA-Japan, Inc., Tokyo, Japan) bearing subcutaneously-implanted (the 2×10E5 cells/0.1 ml RPMI medium were implanted under the skin on the back) squamous cell carcinoma (SCC) was given the oral administration of TiO2/5-ALAparticles suspension. After 4 hours, the tumor tissue (5×7×7 mm3) was removed, and the frozen-sectioned slice (10 μm) of the raw tumor tissue was subjected to a Raman microscopic study as well as histological studies with hematoxylin and eosin (H & E) staining after fixation with 70% ethanol aqueous solution. The histological studies were made from the peripheral portion of the tumor.
Animal experiments were done according to the rule and guideline of the animal ethics committee in National University of Fukui to use anesthesia regents (Isoflurane-inhalation, Abbott-Japan Ltd. Co., Tokyo, Japan) at the treatment of mice and at the endpoints (CO2 gas, Uno-Sanso Ltd. Co., Fukui, Japan).
The number of experimental mice in the each 4 groups were 5. The tumor sizes (the approximation volume of elliptical body = [a × b × c] π / 6) were measured every day for 21 days after the treatments using an elaborate digital caliper (Type: Absolute Solar Digimatic Caliper, Mitutoyo Ltd. Co., Niigata, Japan). The average volume of the 5 tumors were calculated to plot on the growth curve with the standard deviations (SD) as shown in Figure 2.
2.4. Cavitation Measurement Changing by Ultrasound Frequency: [Figure 3]
Cavitation effects in the different frequencies (kHz) against 3 kinds of solutions. The base lines [open circle] of the cavitation at (US only), (US + TiO2)=[closed black circle] and at (US + TiO2+ALA)=[closed red triangle] groups were normalized at the average intensities of 5 times experiments, respectively as shown in Figure 3.
Spectrum Analyzer (Tektronix 3026 type, Tektronix Japan Ltd. Co., Tokyo, Japan), Probe (Hydrophone) of the cavitation (TC4038 type, RESOV Ltd. Co., Slangerup, Denmark), Source of ultrasonic irradiation (1510J-MT type, Branson Ultrasonics Ltd. Co., Connecticut, USA), The 1 ml sample aqueous solution was irradiated in the degassed water bath. The hydrophone probe was set in the sample tube and the cavitation signal (acoustic noise) was detected by the spectrum analyzer.
2.5. ESR (Electron Spin Resonance) Measurement with Spin-trapping Method: [Figure 4]
The typical 1:2:2:1 spectrum pattern of spin-trapping agent, 150 mM DMPO (5,5′-dimethyl-1-pyroline-N-oxide, LABOTEC, Ltd. Co., Tokyo, Japan)-OH radical adduct, was observed by ESR (Model-JES-RE1X, JEOL Co. Ltd., Tokyo, Japan) measurement in 2 different solutions.
(1) closed circle: saline solution, and (2) open circle: 0.2%-TiO2 in 1 mM 5-ALA saline solution after the irradiation (50 W) by ultrasound at 42 kHz as shown in Figure 4. The sample solutions (1 ml) were sonicated for 1, 2, 5, 10 min in an ultrasonic field in 13 × 100 mm disposable pyrex tubes (Corning Inc., Corning, NY, USA) exposed to air and fixed in the center of a sonication bath (Bransonic 1510, frequency = 42 kHz, 50 W). The power input was determined 50W for comparison, 5 min of sonolysis of 1.0 ml of aqueous argon-saturated Fricke dosimeter solution [55] (ferrous sulfate [1 mM], NaCl [10 mM] and H2SO4 [0.4 M]) under the same conditions gave an absorbance reading of 0.23 +/− 0.01 (mean of five determinations +/− SD) at 302 nm in a 1 cm quartz cell. The power input was determined calorimetrically as 50 W for the radical detection experiments.
2.6. Raman Spectrum Microscope Mapping Image: [Figures 5 and 6]
Raman spectrum microscope (MRS-1000 MTS-model, JASCO Ltd. Co., Tokyo, Japan) was used for the measurement of Raman shift spectrum (Figure 5) and the mapping image (1.45×1.45 mm2) on the cryo-sectioned slice of the raw tumor tissue of the model mouse at 2738 cm−1 peak area excited at 785 nm as same methods as reported by our previous reports [56, 57].
The Raman mapping image was compared with the H & E staining image of the next sliced tissue of the same tumor tissue as shown in Figure 6.
2.7. Partition of TiO2 Nanoparticles before Purification: [Figure-7]
The partition of TiO2 nanoparticles was carried out in the test tubes and observed with different microscopes on the mixed solution (BuOH/AcOH/H2O=4:1:5) after treated by sonication at 42 kHz (70W) for 5 min. A micro-photometer (Type: BH2, Olympus Ltd. Co., Tokyo, Japan) and a transmission electron microscope (TEM; Type: H-7650, Hitachi, Ltd., Co., Tokyo, Japan) were used to observe the nanoparticles interaction with 5-ALA.
2.8. CCC Apparatus and Procedure: [Figure 8]
The CCC system used to separate the nanoparticles interacted with 5-ALA by sonication. The separation column was prepared by winding about 12 m long, 1.6 mm ID PTFE (polytetrafluoroethylene) tubing (Zeus Industrial Products, Orangeburg, SC, USA) onto a circular plastic strip (width: 5 cm; thickness: 1 mm) forming a flat coil with ca 33 ml in capacity. It was immersed in an ultrasound bath (42 KHz) (Branson-1510R-MT Type, Bransonic Inc., Danbury, CT, USA) in a vertical position during the separation. The effluent was pumped with a Waters HPLC pump and monitored with a UV monitor (Uvicord SII, LKB Instruments, Bromma, Sweden) at 280 nm. The chromatogram was recorded with a strip-chart recorder (Pharmacia, Stockholm, Sweden).
A polar two-phase system composed of 1-butanol-acetic acid-water at a 4:1:5 volume ratiowasprepared. The solvent mixture was thoroughly equilibrated in a separator funnel and the two phases were separated shortly before use. The separation of TiO2 particles was performed in the Laboratory of Bio-separation Technology, NHLBI, NIH as follows: the column was first completely filled with the upper organic stationary phase followed by sample injection through the sample port. Then, the aqueous mobile phase was eluted through the column at a flow rate of 1 ml/min, while the effluent was continuously monitored at 280 nm with the UV detector and fractionated into test tubes at 4 ml/tube. The temperature of the ultrasound bath was manually controlled at 22–26°C by adding ice as shown in Figure 8.
2.9. Analysis of CCC Fractions: [Figure 10]
The distribution of TiO2 nanoparticles mixed with 5-ALA, fraction No. 5, 6, and 7 (0.5 ml saline solution), separated by CCC were measured by a scatter method instrument [Zetasizer Nano ZS-type, Malvern Institutes Ltd., Worcestershire, UH] in the National Institute of Advanced Industrial Science and Technology (NAIST) in Nagoya, Japan as shown in Figure 10. The measured sample solutions of the fraction No. 5, 6, and 7 were suspended in 0.5 ml saline solution.
3. Results and Discussion
We had been studied of PDT using 5-ALA as a precursor, protoporphyrin-IX (Pp-IX) of hem [59] which had used as a photosensitizer and a fluorescent indicator of cancer margin of glioblastoma during the neurosurgical operation in clinical uses of Japan. Pp-IX has a good affinity of the tumor tissue. We had the plan to use 5-ALA as the carrier of nanoparticles into the tumor tissue if the affinity interactions of them would be given after the modification by the sonication of them with ultrasound. It will be very important thing in the biological nano-sciences to apply using the nanoparticles for the cancer treatment. Hence, we started to build up the instrument of ultrasound irradiation against the tumor model as shown in Figure 1. The mixed solution of 0.2%-titanium oxide particles and 10 mM 5-ALA were oral-administrated into the model mice immediately after the sonication (42 kHz, 70 W) for 5 min at 3 hr before the combined treatments of SDT and PDT as shown in Figure 2.
3.1. Combination Therapy of PDT and SDT: [Figure-2]
The SCC (Squamous Cell Carcinoma) tumour tissues were irradiated by laser light (635 nm, 150 mW/cm2 for 1,000 sec = 150 J/cm2) before the ultrasonic treatment (1 MHz, 70 W for 10 min) using the instrument of Figure 1. (1) Green curve indicates the control group without any treatments and it highlights the exponential growing of the tumour sizes. (2) Blue curve represents single PDT after 3 hr of the oral administration of 0.1 mM 5-ALA saline solution (0.2 ml) without 0.2% titanium oxide particles. There was a mild anti-tumour effect by PDT. (3) Pink curve shows single PDT after 3 hr of the oral administration of 0.1 mM 5-ALA saline solution (0.2 ml) immediately mixing with 0.2% titanium oxide particles suspended in a phosphate aqueous solution diluted with saline solution. It was considered that the titanium nano-particles may trigger in the anti-tumour effect in 5-ALA aqueous solution as the nano-particles enhance the photosensitizing effects, which might be caused by the stability in the excited Pp-IX (porphyrin-IX) and effectively transform the excited electron energy or the charge in the excited state. (4) Black curve denotes the combination of PDT and SDT treatments, where SDT after PDT, after 3 hr of the oral administration with 0.1 mM 5-ALA saline solution (0.2 ml) immediately mixing with 0.2% titanium oxide particles suspended in a phosphate aqueous solution diluted with saline solution.
It was observed that there was a synergistic anti-tumour effect of PDT and SDT treatments in the aforesaid fashion. Whereas, only SDT didn’t show any remarkable anti-tumour activity when applied with 5-ALA alone and less than that of PDT when applied after administering 5-ALA/TiO2 mixture (data not shown). Although the missing of the data of only the TiO2 particle with laser light, it was considered that the anti-tumor effect would be a small one as the laser light of 635 nm would not be absorbed by the particle.
3.2. Cavitation Measurement: [Figure-3]
Cavitation effects in the different frequencies (kHz) against 3 kinds of solutions have been shown in Figure 3. The base lines of the cavitation at ultrasound (US) only [open circle], combination of US and TiO2[closed black circle] and combination of US, TiO2and ALA [closed red triangle] groups have been normalized at the average intensities of 5 times experiments, respectively.
The average inside area of these curves of 10 times measurements was compared between those 3 different solutions. It was found that the area in the case of titanium oxide particles only was enhanced by 28%, and in the case of the presence of nano-particles with 5-ALA aqueous solutions the area was enhanced by 36 % at 2f0 (2nd harmonics) and (3/2)f0 (ultra-harmonics) where the fundamental frequency (f0) was about 40 kHz. Accordingly, the yield of the active oxygen species was expected to be increased by these cavitation effects with the collateral presence of 5-ALA and nanoparticles in aqueous solution, even though the frequency and the medium of the tumor tissue were different from that (1 MHz) in animal experiment conditions.
3.3. ESR Measurement of the Active Oxygen Species produced by the Acoustic Cavitation Effects in Aqueous Solutions: [Figure-4]
The typical 1:2:2:1 spectrum pattern of DMPO-OH radical adduct was observed by ESR measurement in 2 different solutions, (1) closed circle: saline solution only and (2) open circle: 0.2%-TiO2 in 1 mM 5-ALA saline solution. It was considered that the yield of the radical adduct of DMPO-OH was increased with the irradiation time of ultrasound in both solutions at 42 kHz. Especially, the increasing speed was very different, finally, the adduct intensity at 10 min in the case of the mixed solution with nanoparticles was 3 times larger than that of the control solution although the frequencies were different from the animal treatments. This enhancement of the OH radical production would be correlated with the cavitation intensity by ultrasound irradiation with nano-particles as shown in Figure 4.
3.4. Distribution of TiO2 Particle Aggregates in the Tumor Tissue Observed by Raman Spectrum Microscope: [Figures-5 and -6]
The mixed aqueous solution of 0.2%-TiO2 particles with 10 mM 5-ALA was oral administrated to the mouse bearing implanted squamous cell carcinoma (SCC). Then, 3 hr later the tumor tissue was removed and embedded into optimum cutting temperature compound medium to freeze at −20°C. The frozen sample was sliced at 10 μm thickness and observed by a Raman spectrum microscope (Figure 5). The sliced tumor tissue was also studied with an optical microscope after H & E histological staining. In Figure 6, the numerous large (3–5 μm size) aggregates of TiO2 particles are seen around blood vessels of the interstitial tumor tissue from the H & E stained image.
The Raman spectra of the 0.2% titanium oxide particles in aqueous solution and in tumor tissue were observed before and after the administration of 1 mM 5-ALA saline solution. However, there is no prior data against the TiO2 powder particles in the Raman’s data bank. We observed that the peaks in the aqueous solution were spread between 2,300 and 3,000 cm−1. The main peak was present at 2,738 cm−1 as same shapes before and after the administration although we have also observed the absorption peak of the nano particle at 353 cm−1 in far-infrared region in the tumor tissue. As the probe spectral peak was found, we could make the image of the distribution of the particles inside the tumor tissue using the mapping method of Raman microscopy as shown in Figure 6.
Histological H & E staining (Right-side image) and a Raman mapping image against TiO2 nanoparticles (Left-side image) of the squamous cell carcinoma (SCC) tumor model tissue are shown in Figure 6. The Raman image was made by mapping for the Raman shift absorption intensities of the peak area at 2738 cm−1 as shown in Figure 5. There were 21,025 mapping points (145×145) for 12 hrs. The Raman spectrum microscopic observation documented that the distributed TiO2particles aggregate at the blood vessel in the tumor tissue.
3.5. Partition of TiO2 Nanoparticles before Purification: [Figure-7]
Distribution of TiO2nanoparticles in the two-phase solvent system composed of 1-butanol-acetic acid-water with and without adding 5-ALA is illustrated in Figure 7. Both test tubes were treated by ultra-sound irradiation (sonication at 42 kHz, 70W for 5 min). As clearly shown, the particles were almost completely distributed into the lower aqueous phase without 5-ALA in the solvent system whereas they were totally distributed at the interface when 5-ALA was added to the solvent system. It was considered that a week interaction of the nanoparticles with 5-ALA to be aggregated after the sonication as shown in the both microscopes (compared with a and b, c and d in photo-microscope, in TEM). The diameter of TiO2 nanoparticles was 50 nm and that of the aggregate with 5-ALA was about 135 nm which was 2.7 times lager in the diameter from the TEM images (c and d).
The CCC separation of the TiO2 particles was performed with the above solvent system by injecting the sample suspension containing 5-ALA at a concentration of 1 mM as shown in Figure 8 instrument.
3.6. CCC Apparatus and Procedure: [Figure 8]
Figure 8 shows CCC apparatus and the procedure of aggregated nanoparticles, separator funnel, flat coiled column, ultra-sound bath, HPLC pump, fraction collector, and recorder parts. The sample solution of 0.5 ml was injected before the lower phase from the column (➔*) to through the HPLC pump (*➔). From the HPLC pump (➔**) was connected with absorbance detector (**➔) at 280 nm to fractionate to the collector as shown in Figure 8. The separation was completed in 2 hours.
3.7. Mechanism of Particle Preparation in CCC: [Figure-9]
Figure 9 schematically shows hydrodynamic process of the two phases in a flat coiled column consisting of two units where the upper phase (clear) is retained in the left loop and the lower mobile phase (shaded) mostly occupies the space in the right loop in each unit. Application of ultrasonic irradiation produces constant carryover of the stationary phase in the mobile phase, resulting in formation of multiple droplets of one phase in the other phase. These droplets carry particle aggregates according to their affinity on the interface. Consequently, larger particles which have higher affinity can move through the column faster than smaller particles which have less affinity at the interface, resulting in a chromatographic separation according to the size of the particles. Without ultra-sonic irradiation the aggregates would stay at the pair of interface of the solvent systems formed on the top and bottom of the loop and remain in the column much longer time without clear separation. These large molecular will be not able to be separated by high performance liquid chromatography (HPLC.
3.8. CCC Separation of TiO2 Particles: [Figure 10]
The effluent of CCC separation was collected in 20 fractions for 2 hours which were pooled into three fractions No. 5, 6 and 7 according to the chromatograph (the chart has not shown). Due to the carryover of the stationary phase, the chromatogram shows multiple small peaks, while fractions No. 5 and 7 showed distinct single peaks. As clearly shown in the diagram, fraction 5 (red) contained the particle aggregates at 4400 nm in diameter and fraction 7 (green) contained the smaller particle aggregates at 31 nm in diameter, while fraction No. 6 (blue) was a mixture of these two fractions. It indicates that the larger particle aggregates are eluted earlier apparently due to their stronger affinity at the interface between the two phases.
It is of a great interest that only two sizes of aggregates were collected, one around 31 nm and the other, 4400 nm in diameter. This suggests that the original TiO2 particles form only two kinds of stable aggregates with 5-ALA. Among these fractions, No. 7 might be suitable for the further animal studies by scaling-up of the present separation system. For the animal application the fractions should be degassed to eliminate 1-butanol and acetic acid followed by the precipitation with ethanol and dispersed in saline prior to the administration to animal tumor model.
4. Conclusions
The efficacy of nano-scale titanium dioxide has been checked against different cancer models [59–60], viral infections [61], bacterial strains [62] as well as for delivering drug to the targeted gene of the damaged cells [63–64]. However, due to the possible toxic effects on the living organisms wide application of TiO2 nanoparticles has been reduced in some extent also [65]. But according to various studies it has been postulated that toxic effects of TiO2 nanoparticles depends on their shape, size, agglomeration state, surface chemistry and above all concentration [66–68]. The most vulnerable tendency of nanoparticles is agglomeration especially under the physiological conditions like neutral pH, water solubility, presence of salts and proteins, etc. [50–51]. Some studies also proved that the use of phosphate buffer can reduce the cytotoxic properties of TiO2 nanoparticles. Thus, for our study we have chosen the nanoparticles of Ti3(PO4)4, which after being absorbed converted into TiO2 to explore its activity as the sono-sensitizer. Moreover, treated mice were under regular observation for checking any sorts of abnormalities like irritation, shin hypersensitivity reactions, breathing problems, unusual weight loss/gain, etc. We didn’t observe any such unusual problems.
Thus our study suggests a great possibility of using titanium oxides along with 5-ALA for applying into the localized tumor tissue (Raman mapping image) even in the advanced stage. Sonodynamic followed by photodynamic therapy (SDT and PDT) can help to get a reasonable anti-tumor effect because the ultrasound can penetrate deeper into the cancer tissue compared with the laser light (635 nm). Our studies also suggested that countercurrent chromatography (CCC) might be a great choice for collecting pure aggregates of the TiO2+5-ALA for the combination therapy of SDT and PDT while avoiding any unpredictableadverse effects due to the agglomeration of the nanoparticles destined into the tumor tissue.
Acknowledgments
The author (N. M.) wishes to express many thanks to Dr. Prof. Shinichiro Umemura for their academic supports. The study was supported by a Grant-in-Aid for Scientific Research and Special study Area: Photo-functional Surface from Japan Society for the Promotion of Science (JSPS). Furthermore, the author like to acknowledge the support by the Stiftelsen fund of Sweden-Japan Cooperative Research Foundation in Kanazawa (Japanese Research Chief: Prof. H. Hisazumi). Finally, the author would like to thank for the companies (Hitachi Ltd. Co., Tokyo; Tayca Co. Ltd., Osaka, and Cosmo Oil Co. Ltd., Tokyo) and these researchers to support various kinds of instruments and reagents used in this research.
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