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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Biomaterials. 2015 Aug 31;72:163–171. doi: 10.1016/j.biomaterials.2015.08.052

Figure 4. CXCL12-mediated NPC polarization is dependent on CXCR4.

Figure 4

A-H, The polarized NPCs in CXCL12 group (E-H) and unpolarized NPCs in the control group (A-D) expressed nestin. I-P, The NPCs’ polarization induced by CXCL12 was blocked by AMD3100. After treating mouse NPCs with AMD3100 (1 μg/ml, 1h), the NPCs were seeded on cover glasses coated with BSA stripes without CXCL12 (I-L Control group) or with CXCL12 (M-P). After 10 min, cells were fixed and stained with phalloidin-rhodamine (red), Nestin (yellow) and DAPI (blue). The cells in the control group appeared no evidence change in morphology (I-H), while the cells in the CXCL12 group no longer polarized towards the CXCL12 stripe (M-P).Scale bar: 20 μm.