Figure 1.

Statistical and functional analyses of the transcriptome responses in Methylococcus capsulatus grown under different copper regimes. (A) Principal component analysis of normalized expression values (Log2) of all differentially expressed transcripts. Individual samples are indicated according to the copper concentration during growth (n = 3); 0 μmol/L copper (●), 0.8 μmol/L copper (■), 1.6 μmol/L copper (___), 5 μmol/L copper (▲). (B) Two‐ way hierarchal clustering analysis of normalized expression values (Log2) of differentially expressed c‐type cytochromes. Y‐axis indicates gene annotation. Individual samples (X‐axis) are labeled with symbols as indicated above. (C) Pie chart showing the distribution of functional classifications of 137 differential expressed genes between soluble methane monooxygenase and particulate methane monooxygenase‐producing cells based on Gene Ontology assignments. Multivariate statistical analyses were performed with the JMP 11 software from SAS Institute Inc., (Cary, NC).