Abstract
The ultrastructure of the nuclear envelope was studied in cardiac muscle cells of 12 patients undergoing cardiopulmonary bypass surgery. Two main types of membrane inclusions, globular and tubular, were observed in the nuclei. The globular type was found in 6 patients. The globular inclusions were about 0-5mu to 1-5mu in diameter and lined by two unit membranes equal in structure to that of the nuclear envelope. The lining was probably always in continuity with the nuclear membranes. Such inclusions contained a granular matrix in which no cytoplasmic organelles could be seen, with the exception of a limited number of ribosomes. The tubular type of nuclear inclusion was observed in the cardiac tissue of 3 patients. These tubules ran in straight or slightly bent segments of about 0-2 mu to 1-6 mu length, interrupted by bendings at obtuse or right angles. The diameter of their lumina measured about 300 A to 800 A. Such tubules were nearly always found in areas of the nucleoplasm rich in heterochromatin. Nuclear pore-like structures were occasionally found in the tubular wall. The tubular wall consisted of a single unit membrane shown to be in continuity with the inner membrane of the nuclear envelope. Since these nuclear aberrances have been observed at all of the stages examined, the possibility is considered that they may represent a specific nuclear response to the process of cardiac hypertrophy.
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- Babai F., Tremblay G., Dumont A. Intranuclear and intranucleolar tubular structures in Novikoff hepatoma cells. J Ultrastruct Res. 1969 Jul;28(1):125–130. doi: 10.1016/s0022-5320(69)90010-0. [DOI] [PubMed] [Google Scholar]
- Bucciarelli E. Intranuclear cisternae resembling structures of the Golgi complex. J Cell Biol. 1966 Sep;30(3):664–665. doi: 10.1083/jcb.30.3.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLYMAN M. J. A new structure observed in the nucleolus of the human endometrial epithelial cell. Am J Obstet Gynecol. 1963 Jun 15;86:430–432. doi: 10.1016/0002-9378(63)90166-2. [DOI] [PubMed] [Google Scholar]
- Ferrans V. J., Jones M., Maron B. J., Roberts W. C. The nuclear membranes in hypertrophied human cardiac muscle cells. Am J Pathol. 1975 Mar;78(3):427–460. [PMC free article] [PubMed] [Google Scholar]
- Jensen H., Engedal H., Saetersdal T. S. Ultrastructure of mitochondria-containining nuclei in human myocardial cells. Virchows Arch B Cell Pathol. 1976 Jul 21;(1):1–12. doi: 10.1007/BF02899139. [DOI] [PubMed] [Google Scholar]
- Middelkamp J. N., Patrizi G., Reed C. A. Light and electron microscopic studies of the guinea pig cytomegalovirus. J Ultrastruct Res. 1967 Apr;18(1):85–101. doi: 10.1016/s0022-5320(67)80233-8. [DOI] [PubMed] [Google Scholar]
- Patrizi G., Middelkamp J. N., Reed C. A. Reduplication of nuclear membranes in tissue-culture cells infected with guinea-pig cytomegalovirus. Am J Pathol. 1967 May;50(5):779–790. [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rake M. O., Ansell I. D., Murray-Lyon I. M., Williams R. Improved liver-biopsy needle. Lancet. 1969 Dec 13;2(7633):1283–1283. doi: 10.1016/s0140-6736(69)90814-9. [DOI] [PubMed] [Google Scholar]
- Saetersdal T. S., Myklebust R., Skagseth E., Engedal H. Ultrastructural studies on the growth of filaments and sarcomeres in mechanically overloaded human hearts. Virchows Arch B Cell Pathol. 1976 Aug 11;21(2):91–112. doi: 10.1007/BF02899147. [DOI] [PubMed] [Google Scholar]
- Sobel H. J., Schwarz R., Marquet E. Nonviral nuclear inclusions. I. Cytoplasmic invaginations. Arch Pathol. 1969 Feb;87(2):179–192. [PubMed] [Google Scholar]
- Terzakis J. A. The nucleolar channel system of human endometrium. J Cell Biol. 1965 Nov;27(2):293–304. doi: 10.1083/jcb.27.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]