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. 2015 Mar 26;3(3):512–519. doi: 10.1111/andr.12022

Figure 1.

Figure 1

Genotyping of the V‐LH determining genetic variants Trp8Arg (A‐>G, rs1800447) and Ile15Thr (A‐>G, rs34349826) after amplification of the targeted LHB region (662 bp). (A) RFLP analysis with NcoI restriction enzyme detecting the alleles of rs1800447 (allele‐specific RFLP bands are underlined): AA‐ homozygote, fragments 473, 96 and 85; GG‐homozygote: fragments 473, and 185 bp. (B) RFLP analysis with BseGI restriction enzyme detecting the alleles of rs34349826 (allele‐specific RFLP bands are underlined): AA‐homozygote, fragments 392, 165, 62 and 43 bp; GG‐homozygote: fragments 436, 165 and 62 bp. The black arrow points to the fragment characteristic to the LHB variant with the G‐allele (V‐LH), ‘AG’ lane indicated PCR‐RFLP pattern from a heterozygote individual. Enzyme digested PCR products were separated on 2.5% agarose gel containing EtBr.