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. Author manuscript; available in PMC: 2016 Apr 18.
Published in final edited form as: Oncogene. 2015 Jun 22;35(13):1632–1642. doi: 10.1038/onc.2015.237

Figure 7.

Figure 7

Gas6 and Axl expression in melanoma cell lines and tissue specimens. A, immunoblots of melanoma (lanes 1-15), MDA-MB-231 (lane 16) and SKOV3 (lane 17) cell lines. B, activation of Axl by overexpressed Gas6 in six melanoma cancer cell lines. C, immunohistochemical staining of the melanoma cell lines A2058 (negative control for Axl and Gas6) and MDA-MB-231 (positive control) and Kidney tissue (positive control for Axl) and representative melanoma tissue sections with Gas6, Axl and ASS1 antibodies as indicated. H&E image is used to rule out the possibility of melanin involvement in IHC staining. D. positive correlation between Gas6 and Axl (left) and between Gas6 and ASS1 mRNA (middle), and between ASS1 and Axl mRNA levels (right) from 103 cases of cutaneous melanoma metastases in TCGA dataset. R, correlation coefficiency. E. elevated expression of ASS1, c-Myc, Axl, and Gas6 mRNA in the primary cell cultures derived from two melanoma patients (SE and GP) before and after failed by ADI treatments as determined by quantitative real-time PCR. Data were presented as mean ± SD of three independent experiments. *p < 0.05 by the student’s t-test.