The effects of resistance exercise training on arterial stiffness in metabolic syndrome

Abstract

Purpose

Arterial stiffness is a strong independent risk factor for cardiovascular disease and is elevated in individuals with metabolic syndrome (MetS). Resistance training is a popular form of exercise that has beneficial effects on muscle mass, strength, balance and glucose control. However, it is unknown whether resistance exercise training (RT) can lower arterial stiffness in patients with MetS. Thus, the aim of this study was to examine whether a progressive RT program would improve arterial stiffness in MetS.

Methods

A total of 57 subjects (28 healthy sedentary subjects; 29 MetS) were evaluated for arterial structure and function, including pulse wave velocity (cfPWV: arterial stiffness), before and after an 8-week period of RT or continuation of sedentary lifestyle.

Results

We found that 8 weeks of progressive RT increased skeletal muscle strength in both Con and MetS, but did not change arterial stiffness in either MetS (cfPWV; Pre 7.9 ± 0.4 m/s vs. Post 7.7 ± 0.4 m/s) or healthy controls (cfPWV; Pre 6.9 ± 0.3 m/s vs. Post 7.0 ± 0.3 m/s). However, when cfPWV is considered as a continuous variable, high baseline measures of cfPWV tended to show a decrease in cfPWV following RT.

Conclusion

Eight weeks of progressive RT did not decrease the group mean values of arterial stiffness in individuals with MetS or healthy controls.

Introduction

Arterial stiffness (AS) is a strong independent predictor of cardiovascular (CV) mortality (Lakatta and Levy 2003). Increased AS is a common feature of metabolic syndrome (MetS) (Donley et al. 2014; Scuteri et al. 2004; Vyssoulis et al. 2010) and is one of the driving forces behind the increased CV risk in patients with MetS. The mechanisms through which MetS increases AS are likely to be multi-factorial. Reduced endothelial function via a reduction in nitric oxide bioavailability, combined with elevated central pressures, increases vascular tone, shifting forces onto the more stiffer collagen fibers (Lakatta and Levy 2003; Scuteri et al. 2004; Stepp 2006; Wilkinson et al. 2004). Hypertension increases collagen deposition and decreases elastin (Kato et al. 1991; Levy et al. 1993; Rattazzi et al. 2012). Further, a hyperglycemic state likely results in an increased accumulation of advanced glycation end products and the non-enzymatic glycation of matrix proteins, increasing AS in MetS (Emre et al. 2009). A stiffer arterial system can increase central systolic blood pressure (cSBP) leading to increasing cardiac work and decreasing diastolic blood pressure, which in turn can decrease coronary blood flow (Lakatta and Levy 2003). Therapeutic interventions aimed at lowering AS in individuals with MetS are increasingly being seen as important to slow or reverse the progression of CV disease.

Resistance training (RT) is a popular form of exercise that has been shown to exert beneficial effects on muscle mass (improved insulin sensitivity and weight loss), while reducing the risk of sarcopenia, osteoporosis, and frailty (Ibanez et al. 2005). RT is also recognized and recommended by the American Heart Association as a beneficial therapy to help improve the CV health of diseased populations (Williams et al. 2007). Although we have previously shown that aerobic exercise training for 8 weeks reduces AS in subjects with MetS (Donley et al. 2014; Fournier et al. 2015), what effect RT has on AS in MetS is unknown. Previous studies in healthy young and middle-aged subjects suggest that AS increases after RT (Cortez-Cooper et al. 2005; Miyachi et al. 2003, 2004), while other studies showed no change or a decrease with RT (Casey et al. 2007; Heffernan et al. 2009; Rakobowchuk et al. 2005). Furthermore, studies conducted using healthy young or middle-aged obese individuals with fairly healthy measures of AS, have shown no alterations in central AS or augmentation index (an indirect measure of AS) (Croymans et al. 2014; Ho et al. 2012). These differences likely arise from the variations in training programs with high intensity or failure training eliciting increases in AS and beneficial or no change coming from progressive moderate programs. Importantly, it remains unknown in individuals with MetS (but still symptoms for overt CV disease or diabetes absent) whether RT would be beneficial or have an adverse effect on AS. Therefore, the purpose of this study was to examine the effects of RT on AS in individuals with MetS (without overt CV disease or diabetes). Further, we wanted to establish if the RT response to AS differed in those with MetS compared to healthy middle-aged subjects. We hypothesized based on data from similar training programs that a progressive 8-week RT program for those with MetS would not alter AS, but significantly improve skeletal muscular strength and aerobic capacity. We also hypothesized that the RT response would be similar between MetS and healthy controls.

Methods

Study population

Initially, 30 healthy controls and 30 subjects with MetS consented to participate in the study. Individuals were randomly assigned using opaque envelopes into either an RT intervention group or a non-exercised control (Con) group for 8 weeks. However, three subjects (two healthy, one MetS) dropped out due to personal reasons or injuries sustained outside of the research protocol. In total, 28 healthy controls (46 ± 11 year; 72 % female) and 29 MetS (51 ± 12 year; 70 % female) subjects completed the 8-week study with ≥90 % adherence (completed at least 22 of 24 workouts) to the exercise protocol (Fig. 1). The healthy and MetS Con groups maintained their normal lifestyle (time control) for 8 weeks. We selected 8 weeks of RT, because we have previously shown that 8 weeks of aerobic exercise training is beneficial in lowering AS by approximately 10 % (Donley et al. 2014), which may reflect a lowering of CV disease events by approximately 14 % (Vlachopoulos et al. 2010).

Fig. 1.

Flowchart of study and subjects

The healthy and MetS subjects were free from overt CV disease and diabetes, as determined by a medical history, physical examination, and a normal resting and exercise electrocardiogram. Furthermore, none of the participants were current or former smokers. MetS was defined according to the updated National Cholesterol Education Program, Adult Treatment Panel III (Huang 2009) composed of three out of the following five components:

1. obesity (waist: men >102 cm, women >88 cm).

2. low high-density lipoprotein cholesterol (men <1.03 mmol/L; women <1.29 mmol/L).

3. hypertriglyceridemia (>1.7 mmol/L).

4. elevated glucose (>5.6 and <7.0 mmol/L).

5. elevated blood pressure (BP) (130/85 mmHg or use of hypertensive medications).

Additionally, exclusion criteria included type 2 diabetes mellitus (hemoglobin A1c >6.5 %, use of diabetic medications, or doctor’s diagnosis), pulmonary disease, angina, atrial fibrillation, aortic stenosis, anemia, myocardial infarction, stroke, or coronary revascularization. Subjects who participated in regular exercise, defined as >30 min, 3 times/week were also excluded.

This study was approved by the West Virginia University Institutional Review Board, and all procedures were in accordance with the ethical standards of the 1964 Helsinki Declaration and its later amendments. All subjects provided informed written consent prior to participation.

Study design

Assessments were performed between 7:00 and 10:00 AM, in a quiet, temperature-controlled room after a 12-h fast and abstinence from alcohol, caffeine, and vitamins. Blood pressure and cholesterol medications were withheld 24 h before assessments. On completion of the anthropometric assessments and after a minimum of 15 min of quiet supine rest, subjects underwent supine measures of arterial structure and function followed by a blood draw. All pre- and post-measurements were performed at the same time of day for each subject and at least 48 h after the last exercise session to avoid the immediate effects of a single bout of exercise.

Body anthropometry

Height and weight, along with waist and hip circumference, were measured using standard laboratory procedures. Fat distribution was assessed by measuring the waist circumference at the site of the smallest circumference between the rib cage and the iliac crest, with the subjects in a standing position. Body composition was calculated from body volume by the BodPod (Life Measurement, Concord, CA). Body mass index was calculated as: weight (kg)/height² (m).

Metabolic indices

Venous blood sampling was obtained in the morning after a 12-h overnight fast. Post-training venous samples were collected at least 48 h after the last exercise session. Plasma obtained from blood sampling was analyzed at West Virginia University Hospital’s central laboratory in Morgan-town, West Virginia. Total cholesterol, HDL, triglycerides, and glucose were determined in plasma (lithium heparin, Becton Dickenson Plasma Separator Tubes) using Beck-man-Coulter (CA, USA) DxC automated chemistry analyzers. Total cholesterol was measured using a cholesterol esterase/cholesterol oxidase/peroxidase-driven, timed-end-point method (coefficients of variation <7 %). HDL cholesterol was measured using a cholesterol esterase/cholesterol oxidase/peroxidase-driven, timed-endpoint assay with automated initial homogeneous solubilization step (coefficients of variation <7 %). Triglycerides were measured using a lipase/glycerol kinase/glycerophosphate oxidase/horseradish peroxidase-driven, timed-endpoint method (coefficients of variation 5–7 %). Glucose was measured electrochemically using a glucose oxidase/catalase/molybdate-driven oxygen rate method (coefficients of variation <5 %). Glycated hemoglobin (A1c fraction) was measured in whole blood (K₂-EDTA, Becton Dickenson) using a BioRad (Hercules, CA, USA) Variant II Turbo High Performance Liquid Chromatography (HPLC) system (coefficients of variation <2 %). Insulin was measured in serum (untreated/ red-top tube, Becton Dickenson) on an Immulite 2000 immunochemistry system (Siemens, USA; coefficients of variation <10 %).

Arterial structure and function

Methods for arterial geometry and function, exercise testing, and metabolic indices are provided in brief below. A detailed description of these methods can be found in our laboratory’s previously published papers (DeVallance et al. 2015; Donley et al. 2014). A single investigator blinded to group allocations performed measurements. The interclass correlation coefficients (ICC) and coefficient of variations for our laboratory have been previously reported (DeVallance et al. 2015) with minimum ICC = 0.94 and maximum variation of 6 % for any measurement.

Arterial geometry

Ultrasound (GE Vivid i, Toronto, Canada) 2-D images of the right common carotid artery (CCA) were obtained containing the carotid bifurcation and >2 cm of the proximal CCA. Images were used to measure lumen diameter and intima–medial thickness (IMT). At rest, the ICC and the coefficient of variation, collected on two separate days, for IMT was: coefficient of variation = 3 %, and ICC = 0.96.

Arterial function

Brachial systolic (bSBP) and diastolic (bDBP) blood pressure was measured with an automated, oscillometric, sphygmomanometer (Critikon Dinamap Compact BP monitor, GE Medical, Tampa, Fla, USA) and pulse pressure (bPP) was calculated from bSBP-bDBP. Pulse wave analysis was performed noninvasively on the radial artery (SphygmoCor system, AtCor Medical, Sydney, Australia). The Sphygmo-Cor system synthesizes a central (ascending aortic) pressure waveform from the radial pressure waveform that does not differ from that of an intra-arterially recorded wave (Chen et al. 1996) using a validated generalized transfer function (Chen et al. 1997) that has good reproducibility under major hemodynamic changes (Sharman et al. 2006). These waveforms were calibrated against brachial mean arterial and diastolic pressure to estimate aortic pressures. From the central pressure wave, central systolic (cSBP), diastolic (cDBP), pulse pressure (cPP), augmented pressure (AP), and the pressure at the forward wave peak, and central augmentation index (AGI) were determined. All measurements were made in triplicate and the mean values used for subsequent analysis. Pressure waveforms with an operator index of >75 % were determined suitable for examination.

Carotid to femoral pulse wave velocity (cfPWV; central arterial stiffness) was measured by applanation tonometry (AtCor Medical, Sydney, Australia). ECG-gated waveforms were sequentially recorded. The aortic distance was calculated as the difference in the distances from the carotid to the suprasternal notch and from the suprasternal notch to the femoral artery or radial artery. The time delay was calculated using a foot-of-the-wave method. Of note, the coefficient of variation and ICC for cfPWV from our laboratory was 3 % and 0.98, respectively.

Aerobic capacity testing

Subjects exercised to exhaustion on a modified Monarch bike to obtain subjects’ maximum aerobic capacity. The pedal speed was set at 50 rpm, and workloads increased by 25 W every 3 min until exhaustion. Peak oxygen consumed (VO_2peak) was measured from breath-by-breath gas analysis (ParvoMedics, Sandy, UT) and verified by subjects achieving a peak HR >85 % predicted max, an RER >1.0, and a BORG scale >18. Subjects had ECG, and heart rate was continuously monitored over the duration of the exercise test, while BP was measured at the end of each exercise stage and prior to maximal effort by experienced exercise physiologists.

Resistance training intervention

MetS and healthy individuals were randomly selected to participate in an RT program for 3 days/week and consisted of six exercises (leg press, chest press, lat pull down, leg curl, shoulder press, and leg extension) on body master weight machines. The exercise intensity was based on individual’s results from a one-repetition maximum (1RM). Subjects completed 1RM testing in the week prior to beginning the RT intervention. Briefly, subjects were familiarized to each machine with three warm-up sets of minimal resistance, followed by sets of five and three repetitions at increasing resistance. Testing finished with one repetition at increasing resistance until the resistance could not be lifted with proper form; this was repeated for all six lifts. RT intensities were increased progressively in 2-week increments: weeks 1–2, 60 % of max; weeks 3–4, 70 % of max; weeks 5–6, 80 % of max; and weeks 7–8, 85 % of max. Subjects were encouraged to complete three sets of 8–12 repetitions to fatigue, but not failure. The load and repetition range comply with those for hypertrophic training set by the American College of Sports Medicine (ACSM) and the National Strength and Conditioning Association and also comply with ACSM’s stance on RT for health in an adult population (Baechle et al. 2008; Kraemer et al. 2002). All training was performed in the Human Performance Lab at West Virginia University and adherence was monitored through exercise logs filled out by staff.

Statistical analysis

Normality of data distribution was evaluated by the Kolmogorov–Smirnov test. Categorical variables were compared by the χ² test. Continuous variables were log transformed as necessary, and pre-exercise variables were compared between groups with a one-way ANOVA with Tukey’s post hoc test. Two-way repeated-measures ANOVA was used to evaluate the effects of exercise training with a time-by-group interaction to determine whether differences existed between group (Mets vs. healthy controls) and condition (RT vs. non-RT). The primary outcome measure was cfPWV and the secondary outcome measure was SBP. The RT group data were pooled and bivariate correlations were run on baseline measurements to the change in cfPWV following the 8 weeks of RT. Further, a backward elimination model was used to determine which of the correlated measurements were predictive of the cfPWV response. All analyses were performed with the statistical package SPSS version 22 (SPSS, Chicago, IL). All values represent mean ± SEM, unless otherwise stated. P ≤ 0.05 was defined as significant. Using G-power (version 3.19.2, Germany), sample size calculation for our primary outcome measure cfPWV requires at least 12 subjects to detect a clinically significant effect of hypertrophic RT on AS, such as a difference in cfPWV of 1.0 ± 1.0 (SD) m/s (Vlachopoulos et al. 2010) assuming a power of 85 % and α error probability of 0.05.

Results

Anthropometric/training

As expected based on study design, MetS groups had significantly elevated levels of blood lipids, abdominal/overall obesity, and blood pressure at baseline compared to the control groups (Table 1). Importantly, there were no significant group differences for sex or age (Table 1). There were also no differences in lipid profile or anthropometric measures between the two respective MetS groups, or between the two Con groups (Table 1, Table 2) with two exceptions: baseline triglycerides were higher in the Con compared to the healthy Con RT group and hemoglobin A1c was high in MetS compared to MetS RT.

Table 1.

Baseline clinical characteristics of the study cohort

	Con		MetS
	Non-RT (n = 12)	RT (n = 16)	Non-RT (n = 16)	RT (n = 13)
Age (years)	44 ± 3	49 ± 3	51 ± 4	51 ± 3
Height (cm)	166 ± 2	166 ± 2	168 ± 2	166 ± 2
Weight (kg)	68 ± 4	67 ± 3	95 ± 6*	87 ± 4*
Body fat (%)	23 ± 3	26 ± 2	38 ± 2*	38 ± 2*
BMI (kg/m2)	24 ± 1	24 ± 0.9	33 ± 2*	32 ± 1*
Sex (% female)	75	75	73	69
Waist (cm)	82 ± 4	82 ± 2	105 ± 3*	97 ± 3*
Hip (cm)	100 ± 3	100 ± 1	117 ± 3*	112 ± 2*
Triglycerides (mmol/l)	1.0 ± 0.1	0.6 ± 0.1	1.7 ± 0.2*	1.7 ± 0.4*
HDL (mmol/l)	1.4 ± 0.1	1.7 ± 0.3	1.1 ± 0.1*	1.2 ± 0.1*
Glucose (mmol/l)	5.3 ± 0.2	5.1 ± 0.2	5.5 ± 0.1*	5.6 ± 0.2*
HbA1c (%)	5.3 ± 0.1	5.1 ± 0.1	5.7 ± 0.1*	5.2 ± 0.1#
Cholesterol (mmol/l)	5.1 ± 0.3	4.6 ± 0.2	4.7 ± 0.3	5.1 ± 0.3
Hypertensive (%)	0	0	44	46
Diabetics (%)	0	0	0	0
Medications
ACE inhibitors (%)	0	0	13	0
ARB (%)	0	0	13	8
Beta-blockers (%)	0	0	6	15
Calcium channel blockers (%)	0	0	6	0
Diuertics (%)	0	0	13	15
Anti-platelet (%)	0	0	0	0
Statin (%)	0	0	19	23

Values are mean ± SEM

BMI body mass index, HDL high-density lipoprotein, HbA1c hemoglobin A1c, ACE angiotensin-converting enzyme, ARB angiotensin II receptor blocker

^*Denotes p ≤ 0.05 compared to both Con groups

^#Denotes significant within group effects

Table 2.

Strength, anthropometric, lipid, and glucose changes following an 8-week intervention

	CON		CON RT		MetS		MetS RT
	Pre	Post	Pre	Post	Pre	Post	Pre	Post
Weight (kg)	68 ± 4	70 ± 5	67 ± 3	67 ± 3	95 ± 6	95 ± 6	87 ± 4	87 ± 4
Body fat (%)	23 ± 3	23 ± 4	26 ± 2	26 ± 2	38 ± 2	38 ± 2	38 ± 2	35 ± 2
Triglycerides (mmol/l)	1.0 ± 0.1	1.0 ± 0.1	0.6 ± 0.1	0.7 ± 0.1	1.7 ± 0.2	1.8 ± 0.2	1.7 ± 0.4	1.5 ± 0.2
HDL (mmol/l)	1.4 ± 0.1	1.2 ± 0.1	1.7 ± 0.3	1.8 ± 0.1	1.1 ± 0.1	1.1 ± 0.1	1.2 ± 0.1	1.2 ± 0.1
Glucose (mmol/l)	5.3 ± 0.2	5.1 ± 0.2	5.1 ± 0.2	5.0 ± 0.1	5.5 ± 0.1	5.4 ± 0.2	5.6 ± 0.2	5.4 ± 0.2
RER	1.1 ± 0.1	1.2 ± 0.1	1.1 ± 0.01	1.1 ± 0.01	1.1 ± 0.02	1.1 ± 0.02	1.1 ± 0.02	1.1 ± 0.02
Peak HR (BPM)	171 ± 4#	173 ± 4#	151 ± 4	150 ± 3	154 ± 4	158 ± 4	153 ± 4	156 ± 5
Strength
Leg press (kg)	–	–	107 ± 7	127 ± 7*	–	–	113 ± 10	159 ± 22*^
% Change	–	–	19		–	–	41
Lat pulldowns (kg)	–	–	45 ± 2	54 ± 3*	–	–	52 ± 4	64 ± 5*
%Change	–	–	20		–	–	22
Bench (kg)	–	–	48 ± 5	68 ± 6*	–	–	65 ± 10	102 ± 15*^
% Change	–	–	42		–	–	57
Leg curl (kg)	–	–	19 ± 2	25 ± 2*	–	–	17 ± 2	28 ± 2*^
% Change	–	–	32		–	–	65
Shoulder press (kg)	–	–	37 ± 3	52 ± 4*	–	–	44 ± 7	68 ± 8*^
% Change	–	–	41			–	55
Leg extension (kg)	–	–	38 ± 3	51 ± 4*	–	–	38 ± 4	51 ± 4*
% Change	–	–	34		–	–	34
Absolute VO2Peak (L/min)	1799 ± 211	1736 ± 209	1501 ± 95	1507 ± 98	1696 ± 165	1664 ± 148	1707 ± 138	1893 ± 199+

Values are mean ± SEM

^*Denotes a significant main effect of RT

^#Denotes group differences

^{^}Denotes a group by time effect for greater increases in MetS RT compared to Con RT, p ≤ 0.05

⁺Represents a group by time interaction for VO_2peak in the MetS RT group following training, p ≤ 0.05

After the exercise intervention, the MetS RT group showed a non-significant 11 % decrease (p = 0.07) in body fat (Table 2). Also, the MetS RT group had a 12 % increase (p ≤ 0.05) in VO_2peak, while no change was found with the Con RT, or among the sedentary (non-exercising) Con or MetS groups (Fig. 2). Muscular strength (measured via 1RM) was increased in all RT subjects for all six lifts; however, MetS RT showed significantly greater improvements in leg press, bench press, leg curl, and shoulder press (Table 2).

Fig. 2.

Peak aerobic capacity (VO_2peak) for all groups, both pre- and post-8 weeks of resistance training. Black bars represent pre values, while gray denotes post values. Hash symbol represents a group-by-time interaction for VO_2peak in the MetS RT group following training p ≤ 0.05

Arterial function and structure

MetS subjects exhibited higher baseline cfPWV (~16 % faster central AS, p < 0.05) compared to healthy subjects, where the combined average for both MetS groups was 7.9 ± 0.4 m/s and the combined average for the healthy subjects was 6.8 ± 0.3 m/s. Further, bSBP, cSBP, bPP, and cPP were higher in the MetS groups compared to the healthy subjects (Table 3). Following RT, central AS (cfPWV), whose baseline values in the MetS RT were greater than Con RT, also remained unchanged with RT (Fig. 3). As shown in Table 3, RT resulted in no changes in any measure of arterial function, namely AP, and AGI.

Table 3.

Arterial health parameters pre- and post-hypertrophic resistance training

	Con		MetS
	Non-RT (n = 12)	RT (n = 16)	Non-RT (n = 16)	RT (n = 13)
IMT (cm)
Pre	0.63 ± 0.04	0.62 ± 0.02	0.71 ± 0.05*	0.68 ± 0.02*
Post	0.64 ± 0.04	0.61 ± 0.02	0.69 ± 0.03	0.66 ± 0.03
CCA diameter (cm)
Pre	0.58 ± 0.02	0.57 ± 0.01	0.60 ± 0.01*	0.61 ± 0.02*
Post	0.59 ± 0.02	0.57 ± 0.01	0.59 ± 0.01	0.61 ± 0.02
bSBP (mmHg)
Pre	118 ± 2	111 ± 3	127 ± 3*	123 ± 3*
Post	116 ± 3	110 ± 2	126 ± 3*	122 ± 3*
bDBP (mmHg)
Pre	75 ± 2	71 ± 2	76 ± 4	72 ± 2
Post	73 ± 2	69 ± 2	77 ± 3	70 ± 2
bPP (mmHg)
Pre	43 ± 3	40 ± 2	50 ± 4*	51 ± 3*
Post	44 ± 3	41 ± 2	50 ± 3*	51 ± 3 *
MAP (mmHg)
Pre	92 ± 2	85 ± 2	95 ± 3*	92 ± 3*
Post	88 ± 2	84 ± 2	95 ± 3*	89 ± 2*
cSBP (mmHg)
Pre	109 ± 3	103 ± 3	115 ± 3*	115 ± 3*
Post	106 ± 3	102 ± 2	116 ± 3*	113 ± 3*
cDBP (mmHg)
Pre	76 ± 2	72 ± 2	78 ± 3	73 ± 3
Post	74 ± 2	70 ± 2	78 ± 3	72 ± 2
cPP (mmHg)
Pre	33 ± 2	32 ± 2	38 ± 3*	42 ± 3*
Post	32 ± 2	33 ± 2	38 ± 3*	41 ± 3*
P1 height (mmHg)
Pre	24 ± 2	22 ± 1	28 ± 2*	29 ± 2*
Post	25 ± 2	23 ± 1	28 ± 1*	30 ± 2*
AP (mmHg)
Pre	8.5 ± 1.5	9.2 ± 1.5	10.0 ± 2.0	12.5 ± 1.6
Post	8.0 ± 1.7	9.9 ± 1.4	10.1 ± 1.8	11.7 ± 1.5
AGI (%)
Pre	25 ± 4	27 ± 3	24 ± 3	28 ± 2
Post	22 ± 5	29 ± 3	25 ± 3	27 ± 2

Values are mean ± SEM; there were no group by time interactions

IMT intima–media thickness, CCA common carotid artery, bSBP brachial systolic blood pressure, bDBP brachial diastolic blood pressure, bPP brachial pulse pressure, MAP mean arterial blood pressure, cSBP central systolic blood pressure, cDBP central diastolic blood pressure, P1 height height of the first pressure peak, AP augmentation pressure, AGI augmentation index

^*Denotes p ≤ 0.05 compared to both Con groups

Fig. 3.

Central pulse wave velocity for all groups, both pre-and post-8 weeks of resistance training. Black bars represent pre values, while gray denotes post values. There was no significant difference in the change from pre to post, but asterisk represents a group difference between MetS vs. Con p ≤ 0.05

The combined baseline average for CCA and IMT was significantly greater in MetS (by 5 and 12 %, respectively) compared to healthy subjects (Table 3). There were no significant changes in CCA or IMT with the RT program in either group, suggesting that 8 weeks of RT produced no changes in arterial structure.

Predisposition to PWV improvement

Since we found no significant changes in arterial structure or function following RT in either MetS or Con groups, we pooled together the RT data from both groups (creating a more comprehensive data set) to evaluate the potential contribution for baseline anthropometric and/or arterial structure/function to change cfPWV following 8 weeks of RT. We found a significant inverse relationship between delta (visit 2–visit 1) cfPWV and baseline measurements of cfPWV (r = −0.67), cSBP (r = −0.52), bSBP (r = −0.48), and IMT (r = −0.45). Next, we included age, sex, and MetS with cfPWV, bSBP, cSBP, or IMT in a backward-linear elimination regression model to determine the predictability of these measures of decreasing cfPWV with RT. Here, we found that baseline cfPWV was the best predictor of changes in cfPWV (r = −0.67, p < 0.01). However, we also ran the backward elimination without cfPWV to determine if any indirect measure was predictive of the change and found both central (r = −0.52, p < 0.01) and peripheral (r = −0.48, p < 0.01) SBP to be predictive.

Discussion

The principal finding in this study is that progressive RT for 8 weeks did not change AS in either the MetS or healthy control groups. However, significant improvement was obtained in muscle strength in both MetS and Con RT groups. Additionally, the aerobic capacity increased in MetS RT. Existing literature reporting on the effects of RT on AS have produced a wide range of results, suggesting increased (Cortez-Cooper et al. 2005; Miyachi et al. 2003, 2004), no change (Casey et al. 2007; Cortez-Cooper et al. 2005; Croymans et al. 2014; Rakobowchuk et al. 2005), or decreased (Beck et al. 2013) effect on AS in either healthy and obese young or middle-aged individuals. Until now, the effects of RT on AS in MetS have been unknown. Given that individuals with MetS are known to have elevated baseline AS leaving them at greater risk of CV events (Donley et al. 2014; Fournier et al. 2014, 2015), any intervention that might increase (i.e., worsen) AS would be detrimental to overall CV health. In a previous study, we have shown that aerobic exercise training (for 8 weeks) decreased cfPWV in MetS (Donley et al. 2014). In contrast, we found that RT did not decrease cfPWV in MetS. It is, however, equally important to note that we found no detrimental effects of RT on AS in either our MetS or healthy control subjects.

Previous studies have established that an increase in AS after RT typically emanated from young healthy men following high-intensity RT (Cortez-Cooper et al. 2005; Miyachi et al. 2003, 2004), with the exception of Casey et al., who found no change in cfPWV in young men following high-intensity RT (Casey et al. 2007). It has been suggested that the type of RT performed is likely to be important when considering its effects on AS. For example, in the study by Miyachi et al., where RT increased arterial dysfunction (AGI and carotid compliance), the RT protocol was a hypertrophic set × repetition scheme with intensity set at 80 % for the entire duration of the study (Miyachi et al. 2004). Further, every third set was completed to failure, which is a high-intensity stimulus. Similarly, Cortez et al. obtained an increase in AS after RT, which incorporated high-intensity failure sets, suggesting that high-intensity or failure RT may be the stimulus for increased AS (Cortez-Cooper et al. 2005), whereas the study by Casey et al. incorporated a progressive RT program and found no effect on AS (Casey et al. 2007). Although the current study utilized multiple similar muscle groups, the total volume of training, as reflected by the number of total sets × total repetitions × resistance, was substantially less than that reported in previous studies (Cortez-Cooper et al. 2005). Further, the current study incorporated a progressive RT program that began at a moderate intensity. Of note, a recent meta-analysis found that this may not be exclusive to RT, suggesting that even higher-intensity, weight-bearing aerobic training can have adverse effects (Montero et al. 2014). It was suggested that the intense muscle contractions needed to complete weight-bearing aerobic training may increase intramuscular pressure, leading to high wall stress in the arteries, resulting in wall remodeling and therefore increased AS (Montero et al. 2014). However, in our study, we implemented a progressive, moderate RT program. Our results in MetS are similar to that identified (i.e., no alterations in cfPWV or AGI) in obese individuals (a single component of MetS) (Croymans et al. 2014; Ho et al. 2012). Therefore, it seems likely that differing styles and intensities of RT interventions may have important consequences on the health and function of the arterial system. This needs to be more carefully evaluated in future studies. Taken together, we believe these data suggest that there is little concern that RT might be detrimental to AS in the context of MetS.

Although we did not find a group change in AS after RT, the backward elimination models suggested that those individuals with a higher baseline cfPWV (using our pooled data) was a predictor of individuals decreasing AS post-RT (Table 4). It is well documented, especially in the Heritage Family study (Bouchard et al. 1999), that not everyone is a ‘responder’ to the exercise training stimulus. A number of articles, which include aerobic training, have looked at demographic and anthropometric predictors of training outcomes (Bouchard 2012; Bouchard and Rankinen 2001; Skinner et al. 2001; Thomas et al. 1985). A recent study identified that RT did not alter AGI in middle-aged obese individuals (Ho et al. 2012). However, once the obese subjects were separated into ‘responders’ and ‘non-responders’ based on a 1 unit decrease in AGI, it was found that their form of RT decreased AGI similar to that seen in the aerobic and combination of RT and aerobic exercise groups. Taken together with our data, this suggests that individuals with the stiffest arteries gained a beneficial lowering of their AS after 8 weeks of progressive RT. However, this correlation may not be unique to RT, as individuals with the highest PWV may benefit from any form of physical activity, because they have the greatest room for improvement. Conversely, does this mean that healthy individuals with ‘normal’ age-associated AS values will not benefit from regular exercise training? We have previously shown that aerobic exercise training in healthy middle-aged individuals significantly lowered AS (Donley et al. 2014). Similarly, other groups have also found that exercise can lower AS in healthy individuals (Currie et al. 2009; Tanaka et al. 2000). The mechanisms behind these improvements likely reflect enhanced endothelial function (a key component of AS), along with changes in the intrinsic properties of the vessel wall (smooth muscle content, collagen and elastin properties). Such adaptations in elastin and collagen have been confirmed in animal studies after exercise interventions (Fleenor et al. 2010; Kingwell et al. 1997; Matsuda et al. 1993; Seals 2014). Another mechanism, which may influence vascular adaptations to RT, is the increased pressures caused by Valsalva-like maneuvers. It is unknown how the specific forces exerted by this maneuver control vascular adaptation to training. As depicted in Fig. 4, those with the most compliant aortas suffered the greatest increase in cfPWV, which may highlight a differing effect of Valsalva. This suggests that adaptations to high aortic pressures in compliant vessels could become stiffer, while vessels that are already stiff are unaffected. To what extent this stimulus causes vascular adaption to RT is unknown and warrants future investigation. Although baseline cfPWV was the strongest predictor of a decrease in PWV via RT, we also identified that baseline bSBP (an important component of AS) was also a good predictor of the drop in cfPWV post-RT. Further, this relationship remained after we removed five individuals on hypertensive medications (data not shown), given that the half-life of hypertensive medications is approximately 2 days. This suggests that in the absence of cfPWV measurements, an individual’s peripheral SBP could be used to determine if they are likely to gain CV improvements from progressive RT.

Fig. 4.

Relationship between baseline cfPWV (a, r = −0.67) and bSBP (b, r = −0.48) to the change in cfPWV elicited by RT

While the benefits of our progressive RT program were limited to gains in skeletal muscle strength, it should be noted that low muscle strength and mass are often associated with the development of MetS (Atlantis et al. 2009). Muscle mass and strength are also found to be highly predictive of a subject’s insulin resistance (Atlantis et al. 2009). As such, RT may also be beneficial in slowing the progression of MetS patients to overt CV disease. Indeed, we saw a clinically relevant trend (albeit, not statistically significant) for decrease in triglycerides in our MetS RT group. We also demonstrated greater increases in muscle strength in MetS compared to Con RT. The early adaptations to resistance training are mainly neurological and may have a greater effect on the slightly (p = 0.1) higher lean mass noted in MetS RT (54 ± 3 kg) compared to Con RT (49 ± 2 kg), and the increase in type II muscle fibers associated with MetS (Miller et al. 2015; Stuart et al. 2013). However, the mechanisms of strength gains in MetS were beyond the scope of this study, so we also cannot rule out other factors. RT-mediated increases in muscle mass are also known to elicit a beneficial shift in body composition (Chilibeck et al. 1998; Hunter et al. 2004; Yarasheski et al. 1999), and here again we observed a trend for decrease in body fat % in MetS with RT. While these changes were not statistically significant, and not enough to change total metabolic risk assessment, continued participation in RT would be expected to produce significant CV benefits (Brown et al. 2001; Cullen 2000; Jacobson et al. 2007). This could mean that longer interventional durations of hypertrophic RT are needed in a MetS population to produce those physiological changes and elicit reduction of CV risk factors that could alter the MetS classification.

Limitations

There are several limitations. First, the sample size for our training and non-training group was modest (n = 13–16) and a subsequent power analysis revealed that we were underpowered for cfPWV (statistical power = 0.10 %). As such, we cannot exclude the possibility of a type II statistical error (i.e., that we have falsely accepted the null hypothesis). However, we have previously shown that a similar sample size was sufficient to identify a significant decrease in cfPWV after aerobic training (Donley et al. 2014). Further, the 0.1 m/s non-significant decrease in cfPWV in the Mets RT group would not be considered clinically relevant even if statistically significant.

A second limitation of the study involves medications. While none of the healthy subjects were on any medications, MetS patients were on hypertensive and/or cholesterol medications, which may have influenced the exercise responses. Although all subjects withheld their blood pressure medications 24 h prior to the physiological measurements, we cannot rule out that the chronic effects of these medications may have impacted the CV parameters. Further, we explored the relationship between baseline PWV and the change in PWV pre- to post-RT removing the five subjects on hypertensive medications, and found that the relationship was not significantly changed (data not shown). Importantly, the MetS population examined in our study is a reflection of individuals with multiple risk factors undergoing medical treatment for those risk factors.

Third, our sample population consisted of women who were pre- (33 %) and post-menopausal (67 %), of which none of the post-menopausal women were on hormonal replacement therapy. No differences or trends were identified in the change in cfPWV following RT in either pre-or post-menopausal groups in either MetS or healthy RT groups (data not shown). As expected, when we examined by menopausal status, post-menopausal women had higher cfPWV values compared to pre-menopausal women (data not shown). Furthermore, previous work has suggested that sex differences are an important variable for RT outcome (Collier et al. 2011). Although our backward regression models showed no effect of sex on changes in cfPWV following RT, a limitation of this study is that we were not powered to examine sex differences. Thus, future research is required to examine the sex-specific, along with the menopausal effects of RT (with various forms of RT) on AS.

Fourth, subjects were asked to maintain their normal lifestyle that included diet and physical activity (for the no RT groups), which were checked via weekly phone calls or during the subjects’ visit to the human performance laboratory. However, we did not utilize food or physical activity logs and as such we recognize that as a limitation in our study design. We did, however, have exercise logs for the RT groups.

Finally, the short duration of exercise training (8 weeks) may have been insufficient to alter AS. We chose to examine 8 weeks of exercise training as we have previously shown beneficial effects of 8 weeks of aerobic training in MetS on AS. Based on the varying AS responses to RT found in the existing literature, we also cannot exclude the possibility that longer duration RT may not also ultimately alter AS, in either a positive (i.e., decreased PWV) or negative (i.e., increased PWV) manner relative to CV health. Thus, longer exercise training programs are important to fully understand the role that exercise training has on improving CV structure/function in patients with MetS.

In summary, in our population of MetS, 8 weeks of progressive RT produced significant changes in muscle strength, but did not significantly alter the indices of arterial structure and function related to the assessment of arterial stiffness. Our backward regression modeling found that those with the stiffest vessels had the largest reduction in PWV following the 8 weeks of RT, suggesting that significant changes in AS in association with RT (or perhaps any form of) exercise may only be fully realized by individuals with arterial dysfunction.

Abbreviations

1RM

One repetition maximum

AGI

Augmentation index

ANOVA

Analysis of variance

AP

Augmented pressure

AS

Arterial stiffness

bDBP

Brachial diastolic blood pressure

bPP

Brachial pulse pressure

bSBP

Brachial systolic blood pressure

CCA

Common carotid artery

cfPWV

Carotid to femoral pulse wave velocity

Con

Control

cSBP

Central systolic blood pressure

CV

Cardiovascular

DBP

Diastolic blood pressure

HDL

High-density lipoprotein

ICC

Interclass correlation coefficients

IMT

Intima-medial thickness

MetS

Metabolic syndrome

RT

Resistance training

SEM

Standard error of the mean

VO2peak

Peak oxygen consumption