Skip to main content
NCBI home page
British Heart Journal logoLink to British Heart Journal
. 1978 Nov;40(11):1251–1256. doi: 10.1136/hrt.40.11.1251

Immunological determination of serum m-AST activity in patients with acute myocardial infarction.

M Inoue, M Hori, Y Nishimoto, S Fukui, H Abe, H Wada, T Minamino
PMCID: PMC483559  PMID: 718764

Abstract

Serum m-AST (mitochondrial isoenzyme of AST) activity in patients with acute myocardial infarction was determined quantitatively by a new immunological technique which is sensitive and easily available. All 31 patients with acute myocardial infarction showed abnormally high levels of serum m-AST (more than 5 KU/ml); the mean serum m-AST activity attained its peak (42.0 +/- 4.9 KU/ml) on the first day after the onset of infarction 5 hours later than that of serum t-AST (total AST) activity in 15 patients whose peak m- and t-AST activities were identified clearly. The individual peak m-AST activity correlated with the total CK released (r = 0.83, n = 15), indicating that the release of m-AST also reflects the infarct size. The ratio of serum m-AST/t-AST increased following myocardial infarction and showed the maximal value (average 25.7%) on the third to seventh day after onset. This ratio in the patients with acute myocardial ifarction was also greater than that in patients with liver disease or with heart failure from causes other than acute myocardial infarction. In the patients who had the additional complication of heart failure and/or cardiogenic shock the ratio was also greater than that is the patients without these hazards. These results indicate that the ratio of serum m-AST/t-AST reflects the severity of the myocardial cellular damage in acute myocardial infarction.

Full text

PDF
1251

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BORST P., PEETERS E. M. The intracellular localization of glutamate-oxaloacetate transaminases in heart. Biochim Biophys Acta. 1961 Nov 25;54:188–189. doi: 10.1016/0006-3002(61)90953-2. [DOI] [PubMed] [Google Scholar]
  2. BOYD J. W. The intracellular distribution, latency and electrophoretic mobility of L-glutamate-oxaloacetate transaminase from rat liver. Biochem J. 1961 Nov;81:434–441. doi: 10.1042/bj0810434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bodansky O., Schwartz M. K., Nisselbaum J. S. Isozymes of aspartate aminotransferase in tissues and blood of man. Adv Enzyme Regul. 1966;4:299–315. doi: 10.1016/0065-2571(66)90024-0. [DOI] [PubMed] [Google Scholar]
  4. Calva E., Aoki K., Delgadillo J., López-Soriano F., Christlieb I. Immunological identification in plasma of mitochondrial and cytoplasmic aspartate transaminase isoenzymes during experimental myocardial infarction. Am Heart J. 1974 May;87(5):614–618. doi: 10.1016/0002-8703(74)90500-6. [DOI] [PubMed] [Google Scholar]
  5. FLEISHER G. A., POTTER C. S., WAKIM K. G. Separation of 2 glutamic-oxalacetic transaminases by paper electrophoresis. Proc Soc Exp Biol Med. 1960 Jan;103:229–231. doi: 10.3181/00379727-103-25469. [DOI] [PubMed] [Google Scholar]
  6. FLEISHER G. A., WAKIM K. G. Presence of two glutamic-oxalacetic transaminases in serum of dogs following acute injury of the liver. Proc Soc Exp Biol Med. 1961 Feb;106:283–286. doi: 10.3181/00379727-106-26310. [DOI] [PubMed] [Google Scholar]
  7. Farmer S. N., Nanji A. M., Boyde T. R. Mitochondrial aspartate transaminase. Lancet. 1970 Feb 28;1(7644):475–475. doi: 10.1016/s0140-6736(70)90871-8. [DOI] [PubMed] [Google Scholar]
  8. Idéo G., De Franchis R., Bellobuono A., Sforzini S., Dioguardi N. Serum cytosolic-mitochondrial enzyme ratio: a tool for the estimation of the severity of acute hepatitis. Z Klin Chem Klin Biochem. 1972 Feb;10(2):74–76. doi: 10.1515/cclm.1972.10.2.74. [DOI] [PubMed] [Google Scholar]
  9. Idéo G., De Franchis R., Bellobuono A., Tornaghi G. Aspartate aminotransferase isoenzymes in human serum in various liver diseases. Enzyme. 1971;12(5):529–536. doi: 10.1159/000459582. [DOI] [PubMed] [Google Scholar]
  10. Inoue M., Hori M., Fukui S., Abe H., Minamino T. Evaluation of evolution of myocardial infarction by serial determinations of serum creatine kinase activity. Br Heart J. 1977 May;39(5):485–492. doi: 10.1136/hrt.39.5.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Killip T., Kimball J. T. A survey of the coronary care unit: concept and results. Prog Cardiovasc Dis. 1968 Jul;11(1):45–52. doi: 10.1016/s0033-0620(68)80010-6. [DOI] [PubMed] [Google Scholar]
  12. MASSARRAT S., LANG N. IMMUNOLOGIE UND ENZYMKINETIK DER GLUTAMAT-OXALACETAT-TRANSAMINASE (GOT) UND DER GLUTAMAT-PYRUVAT-TRANSAMINASE (GPT) 3. UNTERSUCHUNGEN ZUR IMMUNHEMMUNG DER TRANSAMINASEN IM SERUM VON LEBERKRANKEN UND VON PATIENTEN NACH HERZINFARKT. Klin Wochenschr. 1965 Jun 1;43:602–606. doi: 10.1007/BF01879181. [DOI] [PubMed] [Google Scholar]
  13. Morino Y., Tanase S., Watanabe T., Kagamiyama H., Wada H. Large-scale preparation of cytosolic and mitochondrial asparatate aminotransferases from pig heart. J Biochem. 1977 Sep;82(3):847–852. doi: 10.1093/oxfordjournals.jbchem.a131760. [DOI] [PubMed] [Google Scholar]
  14. Murros J., Konttinen A., Somer H. Mitochondrial aspartate aminotransferase in myocardial infarction. Clin Chim Acta. 1973 Oct 12;48(2):241–242. doi: 10.1016/0009-8981(73)90373-2. [DOI] [PubMed] [Google Scholar]
  15. Norris R. M., Whitlock R. M., Barratt-Boyes C., Small C. W. Clinical measurement of myocardial infarct size. Modification of a method for the estimation of total creatine phosphokinase release after myocardial infarction. Circulation. 1975 Apr;51(4):614–620. doi: 10.1161/01.cir.51.4.614. [DOI] [PubMed] [Google Scholar]
  16. PETERSON E. A., WYCKOFF M. M., SOBER H. A. Gradient chromatography of human serum proteins and its application to the examination of "albumin" and "globulin" obtained by ammonium sulfate fractionation. Arch Biochem Biophys. 1961 May;93:428–434. doi: 10.1016/0003-9861(61)90289-2. [DOI] [PubMed] [Google Scholar]
  17. SCHMIDT E., SCHMIDT F. W. ENZYM-BESTIMMUNGEN IM SERUM BEI LEBER-ERKRANKUNGEN. FUNKTIONS-MUSTER ALS HILFSMITTEL DER DIAGNOSE. Enzymol Biol Clin (Basel) 1963;79:1–52. [PubMed] [Google Scholar]
  18. Sobel B. E., Bresnahan G. F., Shell W. E., Yoder R. D. Estimation of infarct size in man and its relation to prognosis. Circulation. 1972 Oct;46(4):640–648. doi: 10.1161/01.cir.46.4.640. [DOI] [PubMed] [Google Scholar]

Articles from British Heart Journal are provided here courtesy of BMJ Publishing Group

RESOURCES